In vivo gas body efficacy for glomerular capillary hemorrhage induced by diagnostic ultrasound in rats.
In vivo gas body efficacy for glomerular capillary hemorrhage induced by diagnostic ultrasound in rats.
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DOI：
10.1109/tbme.2009.2030960
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发表时间：
2010-01
期刊：
影响因子：
--
通讯作者：
Wiggins RC
中科院分区：
文献类型：
--
作者：
Miller DL;Dou C;Wiggins RC
大鼠肾脏肾小球毛细血管出血（GCH）提供了一种模型，用于评估超声诊断或治疗应用中体内气体体的功效。使用两台诊断超声机：一台监测左肾的谐波B模式对比度增强，另一台暴露右肾用于GCH产生。去甲肾上腺素造影剂以1、2、5或10 μl/kg/min输注，输注时间为30、60、120或300 s。右肾暴露在1.5 MHz下2.3 MPa的峰值稀疏压力振幅下。循环剂量的估计与一个简单的模型的代理稀释和气体体损失。对于以5 μl/kg/min输注300 s，左肾图像亮度增加至平台，估计循环剂量为6.4±1.3 μl/kg，组织学切片中无GCH。以1 s图像间隔暴露右肾，估计循环剂量降至1.3±0.3 μl/kg，并诱导68.4% GCH。剂量和持续时间的增加使每个气体体的治疗效果迅速降低。由于小动物体内的高气体体破坏，大鼠体内的有效药剂剂量可能会大大降低，这使得对人类治疗的类似条件的预测变得复杂。
Glomerular capillary hemorrhage (GCH) in rat kidney provided a model for assessing in vivo gas body efficacy in diagnostic or therapeutic applications of ultrasound. Two diagnostic ultrasound machines were utilized: one monitored the harmonic B-mode contrast-enhancement of the left kidney and the other exposed the right kidney for GCH production. Definity contrast agent was infused at 1, 2, 5 or 10 μl/kg/min and infusion durations were 30, 60, 120 or 300 s. Exposure of the right kidney was at a peak rarefactional pressure amplitude of 2.3 MPa at 1.5 MHz. The circulating dose was estimated with a simple model of agent dilution and gas body loss. For 300 s infusion at 5 μl/kg/min, the left kidney image brightness increased to a plateau with an estimated 6.4±1.3 μl/kg circulating dose with no GCH in histological sections. Exposure of the right kidney with a 1 s image interval reduced the estimated circulating dose to 1.3±0.3 μl/kg and induced 68.4 % GCH. Dose and duration increases gave rapidly diminishing treatment effectiveness per gas body. The effective in vivo agent dose in rats can be reduced greatly due to high gas body destruction in the small animal, complicating predictions for similar conditions of human treatment.