SLC4A transporters.
SLC4A transporters.
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DOI：
10.1016/b978-0-12-394316-3.00003-x
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发表时间：
2012
影响因子：
--
通讯作者：
Choi I
中科院分区：
文献类型：
--
作者：
Choi I
SLC4A基因家族蛋白包括碳酸氢盐转运蛋白，这些转运蛋白通过质膜移动HCO3 -，并调节细胞内pH和酸碱等量物的跨上皮运动。这些转运体包括Cl/HCO3交换体、电致Na/HCO3共转运体、电中性Na/HCO3共转运体和Na+驱动的Cl/HCO3交换体。体外和体内对碳酸氢盐转运体的研究已经证明了它们在细胞和全身水平上对酸碱稳态的生理重要性。结构/功能分析的最新进展也为调控、离子易位和蛋白质拓扑结构提供了重要的结构域或基序信息。本章的重点是离子转运的分子机制以及相关的结构方面，从特定残基的诱变和嵌合结构。结构/功能研究有助于理解离子底物通过转运体移动的机制。本章还介绍了SLC4A1 （AE1）和SLC4A4 （NBCe1）转运体的结构。最后，由于一些SLC4A转运体与细胞中的其他蛋白质协同存在，因此简要讨论了与蛋白质-蛋白质相互作用相关的结构特征。
SLC4A gene family proteins include bicarbonate transporters that move HCO3− across the plasma membrane and regulate intracellular pH and transepithelial movement of acid–base equivalents. These transporters are Cl/HCO3 exchangers, electrogenic Na/HCO3 cotransporters, electroneutral Na/HCO3 cotransporters, and Na+-driven Cl/HCO3 exchanger. Studies of the bicarbonate transporters in vitro and in vivo have demonstrated their physiological importance for acid–base homeostasis at the cellular and systemic levels. Recent advances in structure/function analysis have also provided valuable information on domains or motifs critical for regulation, ion translocation, and protein topology. This chapter focuses on the molecular mechanisms of ion transport along with associated structural aspects from mutagenesis of particular residues and from chimeric constructs. Structure/function studies have helped to understand the mechanism by which ion substrates are moved via the transporters. This chapter also describes some insights into the structure of SLC4A1 (AE1) and SLC4A4 (NBCe1) transporters. Finally, as some SLC4A transporters exist in concert with other proteins in the cells, the structural features associated with protein–protein interactions are briefly discussed.
用vallyomycin或gramicidin处理的人红细胞中DIDS不敏感的氯化物电导的电辐射，屏障和门控分析。
影响因子：
3.8
作者：
Freedman, J C;Novak, T S
通讯作者：
Novak, T S