Low cost production of 3D-printed devices and electrostimulation chambers for the culture of primary neurons
Low cost production of 3D-printed devices and electrostimulation chambers for the culture of primary neurons
复制标题
DOI：
10.1016/j.jneumeth.2015.05.001
复制
发表时间：
2015-08-15
影响因子：
3
通讯作者：
Stagi, Massimiliano
中科院分区：
文献类型：
--
作者：
Wardyn, Joanna D.;Sanderson, Chris;Stagi, Massimiliano
初级神经元的分析是神经生物学许多领域的基本要求。然而，可用于培养原代神经元的商业系统的范围在功能上是有限的，并且这些设备的费用是探索性和大规模研究的障碍。这是特别相关的，因为初级神经元通常需要不寻常的几何形状和专门的涂层以实现最佳生长。幸运的是，最近3D打印的革命提供了生成定制设备的可能性，这些设备可以支持神经元生长并将神经元限制在定义的路径中，从而使神经元生理学的许多方面能够相对容易地进行研究。在本文中，我们详细描述了生产经济实惠的3D打印培养设备所需的系统硬件和软件，这些设备也与活细胞成像兼容。此外，我们还描述了如何使用这些设备来生长和刺激神经元内的几何约束的隔间，并提供例子来说明这些协议提供的实验神经生物学的许多方面的实际效用和潜力。(C)2015作者由爱思唯尔公司出版
The analysis of primary neurons is a basic requirement for many areas of neurobiology. However, the range of commercial systems available for culturing primary neurons is functionally limiting, and the expense of these devices is a barrier to both exploratory and large-scale studies. This is especially relevant as primary neurons often require unusual geometries and specialised coatings for optimum growth. Fortunately, the recent revolution in 3D printing offers the possibility to generate customised devices, which can support neuronal growth and constrain neurons in defined paths, thereby enabling many aspects of neuronal physiology to be studied with relative ease. In this article, we provide a detailed description of the system hardware and software required to produce affordable 3D-printed culture devices, which are also compatible with live-cell imaging. In addition, we also describe how to use these devices to grow and stimulate neurons within geometrically constrained compartments and provide examples to illustrate the practical utility and potential that these protocols offer for many aspects of experimental neurobiology. (C) 2015 The Authors. Published by Elsevier B.V.