A novel device for continuous long-term electroencephalogram recording and drug administration in mice with a nice, powerful and sophisticated wired system
A novel device for continuous long-term electroencephalogram recording and drug administration in mice with a nice, powerful and sophisticated wired system
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DOI：
10.1016/j.jneumeth.2017.04.005
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发表时间：
2017-07-15
影响因子：
3
通讯作者：
Takahashi, Masami
中科院分区：
文献类型：
--
作者：
Watanabe, Shigeru;Saito, Masanori;Takahashi, Masami
背景资料：为了阐明癫痫发生和癫痫成熟的机制，并开发新的AEDs，这是必不可少的管理各种药物，并检查其对EEG的影响，在一个长时间的observation.New方法：我们构建了一个装置，连续测量脑电图（EEG）和抗癫痫药物的输注，在一段较长的时间内在移动的小鼠。该系统包括滑环和转环，分别防止记录电缆和输注管扭曲。我们介绍了三个武器，滚珠轴承，和稳定的框架旋转滑环和旋转，只有一个小的作用力，并促进滑环和旋转的开始。结果：连续脑电图记录成功地进行了长达63天的99只小鼠，共1872天的脑电图数据。连续药物输注与连续EEG记录成功地进行了长达22d.Comparison with Existing Methods（s）：我们的系统是上级优于目前的系统，在连续药物输送在长期EEG记录在移动mouse.Conclusions：我们的设备将是非常有用的长期EEG记录和药物应用在移动mouse. (C)2017爱思唯尔B.V.保留所有权利。
Background: To elucidate mechanisms of epileptogenesis and epileptic maturation, and to develop new AEDs, it is indispensable to administer various drugs and to examine their effects on EEG over a long period of observation.New Method: We constructed a device for the continuous measurement of electroencephalography (EEG) and the infusion of anti-epileptic drugs over a prolonged period of time in moving mice. The system includes a slip ring and a swivel to prevent twisting of the recording cable and infusion tube, respectively. We introduced three arms, ball bearing, and stabilizing frame to rotate the slip ring and swivel with only a small applied force, and to facilitate the start of rotation of the slip ring and the swivel.Results: Continuous EEG recording was successfully performed for up to 63 days in 99 mice, for a total of 1872 days of EEG data. Continuous drug infusion with continuous EEG recording was successfully performed for up to 22 days.Comparison with Existing Method(s): Our system is superior to current system in continuous drug delivery during long-term EEG recording in moving mouse.Conclusions: Our device will be quite useful for long-term EEG recording and drug application in moving mice. (C) 2017 Elsevier B.V. All rights reserved.