Vesicular Glutamate Transporter 3-Expressing Nonserotonergic Projection Neurons Constitute a Subregion in the Rat Midbrain Raphe Nuclei
Vesicular Glutamate Transporter 3-Expressing Nonserotonergic Projection Neurons Constitute a Subregion in the Rat Midbrain Raphe Nuclei
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DOI：
10.1002/cne.22237
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发表时间：
2010-03-01
影响因子：
2.5
通讯作者：
Kaneko, Takeshi
中科院分区：
文献类型：
--
作者：
Hioki, Hiroyuki;Nakamura, Hisashi;Kaneko, Takeshi
我们先前报道说，大约80％的囊泡谷氨酸转运蛋白3（VGLUT3）阳性细胞表现出对5-羟色胺的免疫反应性，但其他大鼠中脑raphe核（例如背侧（DR）和中间raphe nuclei（MNR）中，其他细胞为阴性。在本研究中，为了研究DR和MNR中表达vglut3表达非素能神经元的精确分布，我们对VGLUT3和色氨酸羟化酶2进行了双荧光原位杂交（TPH2）。根据VGLUT3和TPH2 mRNA信号的分布，我们将DR分为六个子区域。在MNR和Rostral（DRR）中，vglut3和TPH2 mRNA信号的DR经常被共定位（约80％）。在DR（DRDC）背侧部分的侧翅（DRL）和核心区域中，产生TPH2的神经元主要分布，而大约94％的TPH2产生神经元对VGLUT3 mRNA负阴性。值得注意的是，在DR（DRDSH）背侧部分的壳区域中，大量检测到VGLUT3 mRNA信号，而表达VGLUT3的神经元中约有75％的TPH2 mRNA为阴性。然后，我们检查了血清素能神经元的化学耗竭后，研究了DRDSH中表达VGLUT3表达非素能神经元的投影。在各个大脑区域（例如腹侧盖区域，黑质Nigra pars comcacta，下丘脑核和下丘脑前区域）观察到了投影。这些结果表明，中脑raphe核中表达vglut3的非凝集神经元优先分布在DRDSH中，并通过升轴子通过神经递质谷氨酸来调节许多大脑区域。 J. Comp。神经。 518：668-686，2010。（c）2009 Wiley-Liss，Inc。
We previously reported that about 80% of vesicular glutamate transporter 3 (VGLUT3)-positive cells displayed immunoreactivity for serotonin, but the others were negative in the rat midbrain raphe nuclei, such as the dorsal (DR) and median raphe nuclei (MnR). In the present study, to investigate the precise distribution of VGLUT3-expressing nonserotonergic neurons in the DR and MnR, we performed double fluorescence in situ hybridization for VGLUT3 and tryptophan hydroxylase 2 (TPH2). According to the distribution of VGLUT3 and TPH2 mRNA signals, we divided the DR into six subregions. In the MnR and the rostral (DRr), ventral (DRV), and caudal (DRc) parts of the DR, VGLUT3 and TPH2 mRNA signals were frequently co-localized (about 80%). In the lateral wings (DRL) and core region of the dorsal part of the DR (DRDC), TPH2-producing neurons were predominantly distributed, and about 94% of TPH2-producing neurons were negative for VGLUT3 mRNA. Notably, in the shell region of the dorsal part of the DR (DRDSh), VGLUT3 mRNA signals were abundantly detected, and about 75% of VGLUT3-expressing neurons were negative for TPH2 mRNA. We then examined the projection of VGLUT3-expressing nonserotonergic neurons in the DRDSh by anterograde and retrograde labeling after chemical depletion of serotonergic neurons. The projection was observed in various brain regions such as the ventral tegmental area, substantia nigra pars compacta, hypothalamic nuclei, and preoptic area. These results suggest that VGLUT3-expressing nonserotonergic neurons in the midbrain raphe nuclei are preferentially distributed in the DRDSh and modulate many brain regions with the neurotransmitter glutamate via ascending axons. J. Comp. Neurol. 518:668-686, 2010. (C) 2009 Wiley-Liss, Inc.