Biodistribution of TNF-alpha-coated gold nanoparticles in an in vivo model system.
Biodistribution of TNF-alpha-coated gold nanoparticles in an in vivo model system.
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体内模型系统中TNF-α涂层金纳米颗粒的生物分布。
DOI：
10.2217/nnm.09.21
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发表时间：
2009-06
期刊：
影响因子：
--
通讯作者：
Bischof JC
中科院分区：
文献类型：
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作者：
Goel R;Shah N;Visaria R;Paciotti GF;Bischof JC
在这项研究中，我们描述了CYT-6091的生物分布，CYT-6091是一种胶体金（Au）基纳米药物，靶向将TNF-α递送到实体肿瘤。将5µg TNF-α包被的CYT-6091单次静脉注射给人前列腺荷瘤或未见肿瘤的裸鼠。收集组织，在特定时间点用原子发射光谱分析金纳米粒子，用ELISA分析TNF-α。CYT-6091的两种成分TNF-α和Au在血液中表现出不同的行为，TNF-α比Au纳米粒子衰变更快。注射后0 ~ 4 h， TNF-α在肿瘤中优先积累。观察到Au在4 - 12小时内优先在肝脏中积聚，并随着时间的推移（4个月）显示出一定的清除。这些数据表明，CYT-6091优先向肿瘤递送TNF-α，并且在TNF-α降解后，肝脏吸收Au，随着时间的推移，Au被缓慢清除。
In this study, we describe the biodistribution of CYT-6091, a colloidal gold (Au)-based nanomedicine that targets the delivery of TNF-α to solid tumors. A single intravenous injection of CYT-6091 coated with 5 µg TNF-α was given to human prostate tumor-bearing or naive (without tumor) nude mice. Tissues were harvested and analyzed at specific time points for Au nanoparticles by atomic emission spectroscopy and TNF-α by ELISA. The two constituents of CYT-6091, TNF-α and Au, exhibited different behavior in blood, with TNF-α showing a faster decay than the Au nanoparticles. Between 0 and 4 h after injection, TNF-α showed a preferential accumulation in the tumor. Au was observed to accumulate preferentially in the liver between 4 and 12 h, and showed some clearance over time (4 months). These data suggest that CYT-6091 delivers TNF-α preferentially to the tumor and that upon TNF-α degradation, the liver takes up Au, which is cleared slowly over time.