Infrared ion spectroscopy: an analytical tool for the study of metabolites
Infrared ion spectroscopy: an analytical tool for the study of metabolites
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DOI：
10.1039/c8an00087e
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发表时间：
2018-04-07
期刊：
影响因子：
4.2
通讯作者：
Polfer, Nicolas C.
中科院分区：
文献类型：
--
作者：
Cismesia, Adam P.;Bell, Matthew R.;Polfer, Nicolas C.
振动离子光谱技术与质谱联用被应用于标准代谢物，作为对未知代谢物结构鉴定的原理验证演示。传统的室温红外多光子解离（IRMPD）光谱技术被证明可区分同量异位和同分异构变体中的化学部分。将这些结果与低温冷却分析物离子的红外光谱进行比较，显示出光谱分辨率提高，从而也改善了对密切相关分子（如异构体）的区分。低温光谱是在最近开发的质量选择低温线性离子阱中实现的，该离子阱具有高灵敏度以及能够同时测量多种分析物的红外光谱的能力。
Vibrational ion spectroscopy techniques coupled with mass spectrometry are applied to standard metabolites as a proof-of-principle demonstration for the structural identification of unknown metabolites. The traditional room temperature infrared multiple photon dissociation (IRMPD) spectroscopy technique is shown to differentiate chemical moieties in isobaric and isomeric variants. These results are compared to infrared spectra of cryogenically cooled analyte ions, showing enhanced spectral resolution, and thus also improved differentiation between closely related molecules, such as isomers. The cryogenic spectroscopy is effected in a recently developed mass-selective cryogenic linear ion trap, which is capable of high sensitivity and the ability to measure the IR spectra of multiple analytes simultaneously.