Perforin-2 Protects Host Cells and Mice by Restricting the Vacuole to Cytosol Transitioning of a Bacterial Pathogen
Perforin-2 Protects Host Cells and Mice by Restricting the Vacuole to Cytosol Transitioning of a Bacterial Pathogen
复制标题
穿孔素 - 2通过限制细菌病原体的液泡到胞质溶胶的转变来保护宿主细胞和小鼠
DOI：
10.1128/iai.01434-15
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发表时间：
2016-04-01
影响因子：
3.1
通讯作者：
Schesser, Kurt
中科院分区：
文献类型：
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作者：
McCormack, Ryan;Bahnan, Wael;Schesser, Kurt
宿主编码的穿孔蛋白-2（由巨噬细胞表达的基因1 Mpeg 1编码）具有成孔MACPF结构域，其降低存在于膜结合区室内的细菌病原体的活力。这里，显示穿孔蛋白-2也限制胞内病原体单核细胞增生李斯特菌的增殖。全身感染后几个小时内，L.穿孔蛋白-2（-/-）小鼠中的单核细胞增多导致急性疾病症状的快速出现。我们继续在培养的穿孔蛋白-2（-/-）细胞中显示，L。单核细胞增生大大加快。出乎意料的是，我们发现在穿孔蛋白-2（-/-）巨噬细胞中，含有李斯特菌的液泡迅速（
The host-encoded Perforin-2 (encoded by the macrophage-expressed gene 1, Mpeg1), which possesses a pore-forming MACPF domain, reduces the viability of bacterial pathogens that reside within membrane-bound compartments. Here, it is shown that Perforin-2 also restricts the proliferation of the intracytosolic pathogen Listeria monocytogenes. Within a few hours of systemic infection, the massive proliferation of L. monocytogenes in Perforin-2(-/-) mice leads to a rapid appearance of acute disease symptoms. We go on to show in cultured Perforin-2(-/-) cells that the vacuole-to-cytosol transitioning of L. monocytogenes is greatly accelerated. Unexpectedly, we found that in Perforin-2(-/-) macrophages, Listeria-containing vacuoles quickly (