Flow cytometric determination of genome size in European seabass (Dicentrarchus labrax), gilthead seabream (Sparus aurata), thinlip mullet (Liza ramada), and European eel (Anguilla anguilla)
Flow cytometric determination of genome size in European seabass (Dicentrarchus labrax), gilthead seabream (Sparus aurata), thinlip mullet (Liza ramada), and European eel (Anguilla anguilla)
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DOI：
10.1051/alr:2005008
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发表时间：
2005-01-01
影响因子：
1.1
通讯作者：
Menu, B
中科院分区：
文献类型：
--
作者：
Peruzzi, S;Chatain, B;Menu, B
用流式细胞仪测定了唇鱼、金鲷、多枝梭子鱼和欧洲鳗鲡的核DNA含量。将男性人白细胞用作内部参考细胞。用含有碘化丙啶（PI）的染色缓冲液将有核鱼红细胞与人白细胞同时染色。相对于男性人白细胞的7.0 pg DNA指定值，估计靶种属的核DNA含量。DNA含量/核（+/- CI）为1.55 +/-0.02pg。labrax，1.90 +/-0.03 pg，用于S. aurata，L. ramada和2.43 ± 0.04pg。安圭拉.种内DNA含量的变化范围从0-9%，平均约4%的所有类群。结果表明，无论是在D。labrax或L.华美达。
The nuclear DNA content in Dicentrarchus labrax, Sparus aurata, Liza ramada, and Anguilla anguilla, was measured by flow cytometric analysis. Male human leukocytes were used as internal reference cells. Nucleated fish erythrocytes were stained simultaneously to human leukocytes with a staining buffer containing propidium iodide (PI). Nuclear DNA contents of target species were estimated in relation to an assigned value of 7.0 pg DNA for male human leukocytes. The DNA content/nucleus (+/- CI) was 1.55 +/- 0.02 pg for D. labrax, 1.90 +/- 0.03 pg for S. aurata, 1.57 +/- 0.02 pg for L. ramada, and 2.43 +/- 0.04 pg for A. anguilla. Intraspecific DNA content variations ranged from 0-9% and averaged around 4% in all taxa. The results did not evidence significant differences in genome size between males and females in either D. labrax or L. ramada.