Effect of interleukin-33 on cementoblast-mediated cementum repair during orthodontic tooth movement
Effect of interleukin-33 on cementoblast-mediated cementum repair during orthodontic tooth movement
复制标题
白细胞介素33对正畸牙移动过程中成牙骨质细胞介导的牙骨质修复的影响
DOI：
10.1016/j.archoralbio.2020.104663
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发表时间：
2020-04-01
影响因子：
3
通讯作者：
Xu, Hui
中科院分区：
文献类型：
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作者：
Dong, Xiaomeng;Feng, Jie;Xu, Hui
目的：本研究旨在揭示白细胞介素-33在成骨水泥细胞介导的骨水泥修复中的作用。方法：采用6 ~ 8周龄C57BL/6小鼠建立正畸牙齿移动模型。免疫组化法检测牙周组织中白细胞介素-33和致瘤性2 （ST2）表达的抑制。在体外，在重组小鼠白细胞介素-33蛋白（rmIL-33）存在下培养成水泥细胞样（OCCM-30）细胞1-14 d。免疫荧光标记ST2表达并定量检测。采用碱性磷酸酶法、茜素红染色法和细胞计数试剂盒-8检测白细胞介素-33对成水泥细胞分化、矿化和增殖的影响。为了进一步明确白细胞介素-33对骨质形成相关蛋白表达的影响，采用western blot检测骨相关转录因子2 （RUNX2）、骨钙素、骨桥蛋白、骨唾液蛋白（BSP）、骨钙素、骨保护素（OPG）和NF-KB配体受体激活因子（RANKL）的表达。结果：高强度正畸负荷诱导小鼠外根尖吸收，增加牙周组织中白细胞介素33的表达。骨水泥中的细胞表达ST2。在OCCM-30细胞中，白细胞介素-33最初下调但随后恢复ST2 mRNA和蛋白水平。白细胞介素-33抑制OCCM-30细胞后期成水泥细胞分化和矿化，抑制RUNX2、osterix、BSP和骨桥蛋白的表达。白细胞介素-33增强OCCM-30细胞中RANKL的表达，降低OPG/RANKL的比值。结论：高强度正畸负荷可诱导牙周组织中白细胞介素-33的表达。白细胞介素-33通过抑制成水泥细胞分化、矿化和成水泥相关蛋白的表达，对水泥形成产生负面影响。
Objective: This study aims to uncover the role of interleukin-33 on cementoblast-mediated cementum repair.Methods: 6-8-week-old C57BL/6 mice were used to establish the model of orthodontic tooth movement. Interleukin-33 and suppression of tumorigenicity2 (ST2) expressions were immunohistochemically detected in the periodontal tissue. In vitro, cementoblast-like (OCCM-30) cells were cultured in the presence of recombinant mouse interleukin-33 protein (rmIL-33) at a 1-14 d time frame. ST2 expressions were immunofluorescently labeled and quantitatively examined. The effects of interleukin-33 on cementoblast differentiation, mineralization and proliferation were examined by alkaline phosphatase, alizarin red staining and cell counting kit-8, respectively. To further clarify the effect of interleukin-33 on cementogenesis-related protein expressions, runtrelated transcription factor 2 (RUNX2), osterix, osteopontin, bone sialoprotein(BSP), osteocalcin, osteoprotegerin (OPG) and receptor activator of NF-KB ligand (RANKL) expressions were examined by western blot.Results: Orthodontic load of high magnitude induces external apical root resorption, and increases interleukin33 expression in the periodontal tissue of mice. Cells in the cementum express ST2. Interleukin-33 initially down-regulates but later recovers ST2 mRNA and protein levels in OCCM-30 cells. Interleukin-33 abates cementoblast differentiation and mineralization, and suppresses RUNX2, osterix, BSP and osteopontin expressions in OCCM-30 cells at the later stage of the culture period. Interleukin-33 enhances RANKL expression, and reduces the ratio of OPG/RANKL in OCCM-30 cells.Conclusion: Orthodontic load of high magnitude induces interleukin-33 expression in the periodontal tissue. Interleukin-33 has a negative effect on cementogenesis via suppressing cementoblast differentiation, mineralization and cementogenesis-related protein expressions.