Evaluation of antioxidant and neuroprotective effect of ethanolic extract of Embelia ribes Burm in focal cerebral ischemia/reperfusion-induced oxidative stress in rats
Evaluation of antioxidant and neuroprotective effect of ethanolic extract of Embelia ribes Burm in focal cerebral ischemia/reperfusion-induced oxidative stress in rats
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DOI：
10.1111/j.1472-8206.2008.00580.x
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发表时间：
2008-06-01
影响因子：
2.9
通讯作者：
Tripathi, C. D.
中科院分区：
文献类型：
--
作者：
Ansari, M. Nazam;Bhandari, Uma;Tripathi, C. D.
抗氧化剂一直是开发用于治疗中风的神经保护剂的研究焦点，中风是一种急性和进行性神经退行性疾病，是世界上第二大死亡原因。事实上，许多草药抗氧化剂已经在体外和体内实验中开发出来，其中一些已经在中风的临床研究中进行了测试。已报道酸藤子具有抗氧化和抗糖尿病作用。除此之外，本研究还探讨了E.茶藨子对大鼠大脑中动脉闭塞（MCAO）所致局灶性脑缺血的保护作用。雄性Wistar白化病大鼠灌胃E.茶藨子提取物（100和200 mg/kg体重; p.o.）30天喂食30天后，所有动物均用水合氯醛（400 mg/kg，腹膜内）麻醉。用4-0缝线闭塞右侧大脑中动脉2 h。2小时后移除缝线以允许再灌注损伤。与假手术组相比，缺血再灌注组大鼠的握力活动和海马和额叶皮质的非酶（还原型谷胱甘肽，GSH）和酶[谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）和谷胱甘肽-S-转移酶（GST）]抗氧化水平显著降低（P < 0.001）。此外，血清乳酸脱氢酶（LDH）和硫代巴比妥酸反应物质（TBARS）在海马和额叶皮质缺血组显着增加与假手术大鼠。此外，乙醇E.与MCAO +溶媒组相比，茶藨子提取物预处理组大鼠握力活动显著增加（P < 0.001），海马和额叶皮质GSH、GPx、GR和GST水平显著升高，血清LDH水平显著降低，海马和额叶皮质TBARS水平显著降低。本研究的数据表明，用乙醇E.茶藨子提取物可增强MCAO诱导的大鼠局灶性脑缺血的抗氧化防御，并具有神经保护活性。
Antioxidants have been the focus of studies for developing neuroprotective agents to be used in the therapy for stroke, which is an acute and progressive neurodegenerative disorder and is the second leading cause of death throughout the world. In fact, many herbal antioxidants have been developed in in vitro and in vivo experiments and some of these have been tested in clinical studies of stroke. Embelia ribes have been reported to have antioxidant and antidiabetic effects. In addition to these effects, this study was designed to investigate the neuroprotective effect of ethanolic extract of E. ribes Burm fruits on middle cerebral artery occlusion (MCAO)-induced focal cerebral ischemia in rats. Male Wistar albino rats were fed ethanolic E. ribes extract (100 and 200 mg/kg body weight; p.o.) for 30 days. After 30 days of feeding, all animals were anaesthetized with chloral hydrate (400 mg/kg, i.p.). The right middle cerebral artery was occluded with a 4-0 suture for 2 h. The suture was removed after 2 h to allow reperfusion injury. Ischemia followed by reperfusion in ischemic group rats significantly (P < 0.001) reduced the grip strength activity and non-enzymatic (reduced glutathione, GSH) and enzymatic [glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST)] antioxidant levels in hippocampus and frontal cortex compared to sham-operated rats. Further, serum lactate dehydrogenase (LDH) and thiobarbituric acid reactive substance (TBARS) levels in hippocampus and frontal cortex were significantly increased in ischemic group compared to sham-operated rats. Furthermore, ethanolic E. ribes extracts pretreatment significantly (P < 0.001) increased the grip strength activity, and GSH, GPx, GR and GST levels in hippocampus and frontal cortex with significant decrease in LDH levels in serum and TBARS levels in hippocampus and frontal cortex compared to MCAO + vehicle group rats. The data from this study suggest that chronic treatment with ethanolic E. ribes extract enhances the antioxidant defense against MCAO- induced focal cerebral ischemia in rats and exhibits neuroprotective activity.