Cadmium-induced changes in vacuolar aspects of Arabidopsis thaliana.
Cadmium-induced changes in vacuolar aspects of Arabidopsis thaliana.
复制标题
镉诱导拟南芥液泡方面的变化。
DOI：
10.1016/j.plaphy.2017.02.017
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Tetsuro Mimura
中科院分区：
文献类型：
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作者：
Shanti S. Sharma;Kotaro Yamamoto;Kohei Hamaji;Miwa Ohnishi;Aya Anegawa;Shashi Sharma;Sveta Thakur;Vijay Kumar;Tomohiro Uemura;Akihiko Nakano;Tetsuro Mimura
我们已经研究了由于镉处理在拟南芥根尖皮层细胞液泡形式的变化，采用GFP融合到液泡膜蛋白质。镉诱导的增强的复杂性与一般的液泡系统的扩展在24小时内是显而易见的。液泡形态的变化依赖于镉的浓度。同时，通过双硫腙染色显示，镉积累在幼苗根系表现出丰富的镉-双硫腙复合物在根尖，根毛和维管系统。为了深入了解SNARE蛋白介导的囊泡融合在镉解毒中的参与，将液泡Qa-SNARE蛋白VAM 3/SYP 22的一对敲除突变体中的镉毒性的大小与野生型中的进行比较。镉的毒性似乎是可比的突变体和野生型。为了在细胞水平上分析镉的影响，我们用镉处理了拟南芥悬浮培养细胞。镉，然而，没有引起的变化，在悬浮培养的细胞中的液泡形式，虽然镉测量与ICP-MS明显采取了进入细胞。镉对拟南芥悬浮培养细胞液泡微粒体V-ATP酶活性无影响。镉处理的细胞的某些代谢产物的水平的变化也不是那么明显，除了谷胱甘肽。研究结果的意义进行了讨论。
We have examined the changes due to Cd treatment in the vacuolar form in root tip cortical cells in Arabidopsis thaliana employing a transformant with GFP fused to a tonoplast protein. A Cd-induced enhancement in complexity with general expansion of vacuolar system within 24 h was evident. The changes in the vacuolar form were dependent on the applied Cd concentrations. Concomitantly, as revealed through dithizone staining, Cd accumulated in the seedling roots exhibiting abundance of Cd-dithizone complexes in root tip, root hairs and vasculature. To get insight into the involvement of SNARE protein-mediated vesicle fusion in Cd detoxification, the magnitude of Cd toxicity in a couple of knock out mutants of the vacuolar Qa-SNARE protein VAM3/SYP22 was compared with that in the wild type. The Cd toxicity appeared to be comparable in the mutants and the wild type. In order to analyze the Cd effects at cellular level, we treated theArabidopsissuspension-cultured cells with Cd. Cd, however, did not induce a change in the vacuolar form in suspension-cultured cells although Cd measured with ICP-MS was obviously taken up into the cell. The V-ATPase activity in the microsomal fractions from vacuoles isolated fromA. thalianasuspension cultured cells remained unaffected by Cd. Changes in the levels of certain metabolites of Cd-treated cells were also not so distinct except for those of glutathione. The significance of findings is discussed.