Properties of the reaction utilizing adenosinetriphosphate for bioluminescence.
Properties of the reaction utilizing adenosinetriphosphate for bioluminescence.
复制标题
利用三磷酸腺苷进行生物发光的反应特性。
DOI：
10.1016/s0021-9258(18)55960-3
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发表时间：
1951
期刊：
影响因子：
--
通讯作者：
W. D. Mcelroy
中科院分区：
文献类型：
--
作者：
W. D. Mcelroy
早期的研究表明，萤火虫提取物中光的产生取决于热不稳定催化剂（荧光素酶）、热稳定的黄绿色荧光化合物（荧光素）、无机离子（Mg++、Co++或Mn++）、氧和三磷酸腺苷（ATP）（1 -3）。磷酸键能对于光产生的必要性表明ATP和发光系统的某些组分之间存在能量偶联反应，可能是荧光素酶复合物。最近，人们已经可以将腺苷三磷酸酶活性从发光反应中分离出来，并在更严格定义的条件下研究ATP的利用。本报告涉及部分纯化系统的性质和ATP用于光产生的动力学和机制。
Earlier studies have shown that the production of light in firefly extracts depends upon a heat-labile catalyst (luciferase), a heat-stable, yellow-green fluorescent compound (luciferin), an inorganic ion (Mg++, Co++, or Mn++), oxygen, and adenosinetriphosphate (ATP)(l-3). The necessity of phosphate bond energy for light production suggests the existence of an energy-coupling reaction between ATP and some component of the luminescent system, presumably the luciferin-luciferase complex. Recently it has been possible to separate the adenosinetriphosphatase activity from the luminescent reaction and to study the utilization of ATP under more rigorously defined conditions. The present report is concerned with the properties of the partially purified system and the kinetics and mechanism of utilization of ATP for light production.