Pentylenetetrazol produces a state-dependent conditioned place aversion to alcohol withdrawal in mice
Pentylenetetrazol produces a state-dependent conditioned place aversion to alcohol withdrawal in mice
复制标题
DOI：
10.1016/j.pbb.2010.02.001
复制
发表时间：
2010-04-01
影响因子：
3.6
通讯作者：
Coon, Laran E.
中科院分区：
文献类型：
--
作者：
Chester, Julia A.;Coon, Laran E.
本研究的目的是确定使用位置调节程序是否可以在小鼠中检测到酒精戒断的厌恶效应，以及 GABA(A) 受体拮抗剂戊四氮 (PTZ) 是否会增加酒精戒断的厌恶效应并增加检测条件性位置厌恶的可能性。受试者是来自针对低酒精偏好选择性培育的特定品系的未接触过酒精的小鼠（LAP1；n = 91），并被分配到三组：酒精戒断组、仅 PTZ 组和 PTZ+酒精戒断组。在四项试验中，小鼠在被放置在独特的地板纹理上进行 30 分钟调节之前 8 小时接受 4.0 g/kg 腹腔 (i.p.) 酒精注射（酒精戒断组、PTZ+酒精戒断组）或生理盐水（PTZ 组）。在这些疗程前五分钟，PTZ 和 PTZ+酒精戒断组中的小鼠接受 PTZ（5.0 mg/kg；腹腔注射），酒精戒断组接受盐水。在干预的几天里，小鼠在被放置在不同的地板纹理之前在同一时间点接受两次盐水注射。通过两次 60 分钟的测试评估后调节地板偏好；第一次测试不涉及药物，第二次测试则取决于状态。戒酒和 PTZ 都没有产生显着的位置调节作用。 PTZ+酒精戒断组在状态依赖性测试中表现出显着的地方厌恶。这些数据表明，PTZ 和酒精戒断的联合刺激特性促进了对酒精戒断的条件性场所厌恶的表达。 (C) 2010 Elsevier Inc. 保留所有权利。
The purpose of this study was to determine if aversive effects of alcohol withdrawal could be detected in mice using the place conditioning procedure and whether the GABA(A) receptor antagonist, pentylenetetrazol (PTZ), would increase the aversive effects of alcohol withdrawal and increase the probability of detecting conditioned place aversion. Subjects were alcohol-naive mice from a specific line selectively bred for low alcohol preference (LAP1; n = 91) and were assigned to three groups: alcohol withdrawal, PTZ alone, and PTZ+alcohol withdrawal. On four trials, mice received either a 4.0 g/kg intraperitoneal (i.p.) injection of alcohol (alcohol withdrawal, PTZ+ alcohol withdrawal groups) or saline (PTZ group) 8 h prior to being placed on a distinctive floor texture for a 30-min conditioning session. Five minutes before these sessions, mice in the PTZ and PTZ+alcohol withdrawal groups received PTZ (5.0 mg/kg; i.p.) and the alcohol withdrawal group received saline. On intervening days mice received two saline injections at the same time points prior to being placed on a different floor texture. Post-conditioning floor preference was assessed in two 60-min tests; the first test was drug-free and the second test was state-dependent. Neither alcohol withdrawal nor PTZ produced significant place conditioning. The PTZ+ alcohol withdrawal group showed a significant place aversion during the state-dependent test. These data suggest that the combined stimulus properties of PTZ and alcohol withdrawal facilitated the expression of conditioned place aversion to alcohol withdrawal. (C) 2010 Elsevier Inc. All rights reserved.