Comparison of an ELISA and two reverse transcription polymerase chain reaction methods for norovirus detection
Comparison of an ELISA and two reverse transcription polymerase chain reaction methods for norovirus detection
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DOI：
10.1016/j.diagmicrobio.2011.04.002
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发表时间：
2011-08-01
影响因子：
2.9
通讯作者：
Deak, Judit
中科院分区：
文献类型：
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作者：
Kele, Beatrix;Lengyel, Gyoergy;Deak, Judit
诺如病毒是不可培养的;因此，ELISA和逆转录聚合酶链反应（RT-PCR）方法被广泛用于其检测。六十一个散发，粪便样本，从各个大学医院病房和门诊病人在匈牙利塞格德，进行了检查。比较了三种方法：两种RT-PCR方法（Argene Calici/Astrovirus Consensus试剂盒和CepheidNorovirus引物和探针组）和一种ELISA方法（IDEIATM Norovirus ELISA Test）。IDEIA（TM）诺如病毒ELISA、Argene试剂盒和Cepheid试剂盒的灵敏度分别为78.9%、92.8%和91.2%，特异性分别为100%、100%和100%。用PCR和ELISA方法检测了52份诺如病毒阳性标本，其中1份为基因组I，其余均为基因组II。虽然ELISA试剂盒的灵敏度低于PCR试剂盒，但它可用于大规模检测。然而，ELISA阴性的暴发应通过RT-PCR方法进行复检。我们的研究结果表明，诺如病毒，主要是诺如病毒属的基因组II，发挥了重要的作用，在急性胃肠炎的爆发和散发病例，不仅在婴儿和幼儿，而且在成人。(C)2011 Elsevier Inc. All rights reserved.
Noroviruses are uncultivable; ELISA and reverse transcription polymerase chain reaction (RT-PCR) methods are therefore widely used for their detection. Sixty-one sporadic, diarrhoeal stool samples from various university hospital wards and from outpatients in Szeged, Hungary, were examined. Three methods were compared: two RT-PCR methods (the Argene Calici/Astrovirus Consensus kit and the Cepheid Norovirus Primer and Probe Set) and one ELISA method (the IDEIA (TM) Norovirus ELISA Test). Sensitivities of 78.9%, 92.8%, and 91.2%, and specificities of 100%, 100%, and 100% were found for the IDEIA (TM) Norovirus ELISA, the Argene kit, and the Cepheid kit, respectively. The PCR and ELISA systems detected 52 norovirus-positive samples, one of which belonged to genogroup I and all the others to genogroup II. Although the ELISA kit has a lower sensitivity compared to the PCR ones, it can be useful for large-scale testing. However, ELISA-negative outbreaks should be retested by RT-PCR methods. Our results suggest that noroviruses, and predominantly genogroup II of the norovirus genera, play an important role in outbreaks and sporadic cases of acute gastroenteritis, not only in infants and young children, but also in adults. (C) 2011 Elsevier Inc. All rights reserved.