Effects of hindlimb unloading on neurogenesis in the hippocampus of newly weaned rats
Effects of hindlimb unloading on neurogenesis in the hippocampus of newly weaned rats
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DOI：
10.1016/j.neulet.2011.12.022
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发表时间：
2012-02-16
影响因子：
2.5
通讯作者：
Ohira, Yoshinobu
中科院分区：
文献类型：
--
作者：
Nomura, Sachiko;Kami, Katsuya;Ohira, Yoshinobu
应用免疫组织化学方法，观察了后肢悬吊（HS）和截肢恢复对新生大鼠海马神经发生的影响。在正常生长过程中，颗粒下带（SGZ）内增殖细胞核抗原阳性（PCNA（+））细胞数量明显减少。然而，无论是HS还是随后的恢复引起额外的变化，PCNA的数量。细胞在正常生长过程中，双皮质素阳性（DCX+）神经元数量逐渐减少。HS导致这些神经元进一步减少。然而，DCX+细胞数量在恢复14天后与年龄匹配的对照组中的水平相同。SGZ内也可见PCNA和DCX双阳性细胞，且其数量不受HS和14天Ambient的影响。因此，HS抑制DCX+神经元的产生，而不影响PCNA（+）细胞在断乳大鼠SGZ。总之，断乳大鼠海马神经发生没有受到严重影响HS，而它显着下降，因为他们已经长大。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Effects of hindlimb suspension (HS) and ambulation recovery on hippocampal neurogenesis of newly weaned rats were studied by using immunohistochemical techniques. The number of proliferating cell nuclear antigen-positive (PCNA(+)) cells in the subgranular zone (SGZ) markedly decreased during normal growth. However, neither HS nor subsequent recovery caused additional changes in the number of PCNA. cells. The number of doublecortin-positive (DCX+) neurons decreased gradually during normal growth. HS resulted in a further decrease in these neurons. However, DCX+ cell numbers became identical to the levels in age-matched controls after 14 days of recovery. PCNA and DCX-double positive cells in the SGZ were also observed, and their cell numbers were not affected by HS and 14-day ambulation. Thus, HS suppressed the generation of DCX+ neurons without affecting PCNA(+) cells in the SGZ of weaned rats. Taken together, hippocampal neurogenesis in weaned rats was not severely affected by HS while it decreased significantly as they had grown. (C) 2011 Elsevier Ireland Ltd. All rights reserved.