Genetic characterization of H5N1 avian influenza viruses isolated in southern China during the 2003-04 avian influenza outbreaks
Genetic characterization of H5N1 avian influenza viruses isolated in southern China during the 2003-04 avian influenza outbreaks
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DOI：
10.1007/s00705-004-0474-9
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发表时间：
2005-06-01
影响因子：
2.7
通讯作者：
Xin, CA
中科院分区：
文献类型：
--
作者：
Wan, XF;Ren, T;Xin, CA
近期亚洲爆发的H5N1禽流感疫情已蔓延至超过8个国家。造成巨大的经济损失，给公众健康带来巨大挑战。在这些爆发期间，我们从中国南方不同农场的鸡中分离出 3 株 H5N1 禽流感病毒 (AIV)，从鸭中分离出一株。我们对这四种 AIV 进行了完整测序。分子表征表明，这些毒株保留了已报道的与病毒毒力和宿主适应相关的 H5N1 AIV 序列特性。系统发育结果表明，其中三个分离株（A/Chicken/Guangdong/174/04 除外）与最近亚洲 H5N1 疫情中分离出的其他 H5N1 AIV 密切相关。 A/Chicken/Guangdong/174/04 的 8 个片段中的 6 个片段（PA 和 M 除外）也与最近 H5N1 疫情中分离出的其他 H5N1 AIV 具有密切的联系。然而，A/Chicken/Guangdong/174/04 的 PA 基因和另一种 H5N1 病毒株与 H6N1 AIV 形成了一个不同的亚群，A/Chicken/Guangdong/174/04 的 M 基因与中国水生物种的一些 H5N1 AIV 具有密切的连锁关系。我们的研究结果表明，在 2003-04 年亚洲禽流感爆发期间，出现了一种新的 AIV 基因型（除了之前报道的基因型外），并且需要对 AIV 进行持续的病毒监测，以监测 A/Chicken/Guangdong/174/04 类 AIV 的进化路径。
The recent H5N1 avian influenza outbreaks in Asia spread over more than 8 countries. It has caused enormous economic loss and grand challenges for the public health. During these breakouts we isolated three strains of H5N1 Avian Influenza Virus (AIV) from chickens and one from duck in different farms of Southern China. We completely sequenced these four AIVs. Molecular characterization demonstrated that these strains retain the reported H5N1 AIV sequence properties relevant to virus virulence and host adaptation. Phylogeny results demonstrated that three of these isolates (except A/Chicken/Guangdong/174/04) were closely linked to other H5N1 AIVs isolated from the recent H5N1 outbreaks in Asia. Six of 8 segments (except PA and M) of A/Chicken/Guangdong/174/04 also shares a close linkage to other H5N1 AIVs isolated from the recent H5N1 outbreaks. However, the PA gene of A/Chicken/Guangdong/174/04 and another H5N1 strain forms a distinct subgroup along with an H6N1 AIV, and the M gene of A/Chicken/Guangdong/174/04 shows a close linkage to some H5N1 AIVs from aquatic species in China. Our findings suggest that a new genotype of AIV (in addition to previous reported ones) was present during the 2003-04 Asian bird flu outbreaks and that continuing virus surveillance of AIVs be conducted to monitor the evolutionary paths of the A/Chicken/Guangdong/174/04-like AIVs.