Coronaviruses from pheasants (Phasianus colchicus) are genetically closely related to coronaviruses of domestic fowl (infectious bronchitis virus) and turkeys
Coronaviruses from pheasants (Phasianus colchicus) are genetically closely related to coronaviruses of domestic fowl (infectious bronchitis virus) and turkeys
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DOI：
10.1080/03079450120106651
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发表时间：
2002-02-01
期刊：
影响因子：
2.8
通讯作者：
Gough, RE
中科院分区：
文献类型：
--
作者：
Cavanagh, D;Mawditt, K;Gough, RE
使用逆转录酶聚合酶链反应 (RT-PCR) 来检查从表现出呼吸道疾病症状的野鸡口腔/鼻拭子中提取的 RNA。使用的寡核苷酸基于传染性支气管炎病毒（IBV）（家禽冠状病毒）的序列。对 IBV 基因组 3' 非翻译区高度保守的 II 区进行的 RT-PCR 在 18/21 庄园的拭子中检测到了冠状病毒。与火鸡 IBV 和冠状病毒相应区域的序列同一性 > 95%。 13/21 的样本中，刺突蛋白基因 S1 区域的部分 RT-PCR 呈阳性。对来自 9 种野鸡病毒的 RT-PCR 产物进行序列分析，结果显示，其中一些病毒彼此之间存在约 24% 的差异，类似于 IBV 不同血清型所表现出的差异程度。对其中一种病毒基因组的进一步分析表明，它含有IBV典型基因3和5，但在哺乳动物冠状病毒的传染性胃肠炎病毒和鼠肝炎病毒组中均不存在。比较不同血清型的 IBV 时观察到，野鸡病毒基因 3 和 5 的核苷酸序列与火鸡和鸡冠状病毒具有相似程度的同一性（约 90%）。这项工作：(a) 证实了冠状病毒存在于野鸡中（事实上，通常存在于患有呼吸道疾病的野鸡中）； (b) 证明他们的基因组在其组织中与 IBV 类似； (c)显示雉鸡冠状病毒组内存在序列异质性，尤其是刺突蛋白基因内。此外，野鸡病毒的基因序列与IBV的基因序列的差异程度相似，因为IBV的一种血清型的序列与另一种不同。根据迄今为止的遗传证据，鸡、火鸡和雉鸡的冠状病毒之间存在非常高的遗传相似性。
Reverse-transcriptase polymerase chain reactions (RT-PCRs) were used to examine RNA extracted from mouth/nasal swabs from pheasants exhibiting signs of respiratory disease. The oligonucleotides used were based on sequences of infectious bronchitis virus (IBV), the coronavirus of domestic fowl. A RT-PCR for the highly conserved region II of the 3' untranslated region of the IBV genome detected a coronavirus in swabs from 18/21 estates. Sequence identity with the corresponding region of IBVs and coronaviruses from turkeys was > 95%. A RT-PCR for part of the S1 region of the spike protein gene was positive with 13/21 of the samples. Sequence analysis of the RT-PCR products derived from nine of the pheasant viruses revealed that some of the viruses differed from each other by approximately 24%, similar to the degree of difference exhibited by different serotypes of IBV. Further analysis of the genome of one of the viruses revealed that it contained genes 3 and 5 that are typical of IBV but absent in both the transmissible gastroenteritis virus and murine hepatitis virus groups of mammalian coronaviruses. The nucleotide sequences of genes 3 and 5 of the pheasant virus had a similar degree of identity (approximately 90%) with those of coronaviruses from turkeys and chickens, as is observed when different serotypes of IBV are compared. This work: ( a) confirms that coronaviruses are present in pheasants (indeed, commonly present in pheasants with respiratory disease); (b) demonstrates that their genomes are IBV-like in their organization; and (c) shows that there is sequence heterogeneity within the group of pheasant coronaviruses, especially within the spike protein gene. Furthermore, the gene sequences of the pheasant viruses differed from those of IBV to similar extents as the sequence of one serotype of IBV differs from another. On the genetic evidence to date, there is a remarkably high degree of genetic similarity between the coronaviruses of chickens, turkeys and pheasants.