Relationship between frequency and deflection angle in the DNA prism
Relationship between frequency and deflection angle in the DNA prism
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DOI：
10.1103/physreve.87.012723
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发表时间：
2013-01-28
影响因子：
2.4
通讯作者：
Dorfman, Kevin D.
中科院分区：
文献类型：
--
作者：
Chen, Zhen;Dorfman, Kevin D.
DNA 棱镜是标准脉冲场电泳方案的改进，可提供连续分离，其中 DNA 偏转的角度取决于其分子量。 DNA 棱镜的标准之字形模型预测，偏转角作为切换场的频率的函数会单调增加，直到达到稳定状态。然而，实验表明，偏转角在高频下衰减到与尺寸无关的值之前达到最大值。使用布朗动力学模拟，我们表明偏转角的最大值与 DNA 的重新定向时间有关，并且高频下偏转角的衰减是由于拉伸不足造成的。偏转角对分子量、转换频率和电场强度的依赖性的一般特征解释了许多实验现象。 DOI：10.1103/PhysRevE.87.012723
The DNA prism is a modification of the standard pulsed-field electrophoresis protocol to provide a continuous separation, where the DNA are deflected at an angle that depends on their molecular weight. The standard switchback model for the DNA prism predicts a monotonic increase in the deflection angle as a function of the frequency for switching the field until a plateau regime is reached. However, experiments indicate that the deflection angle achieves a maximum value before decaying to a size-independent value at high frequencies. Using Brownian dynamics simulations, we show that the maximum in the deflection angle is related to the reorientation time for the DNA and the decay in deflection angle at high frequencies is due to inadequate stretching. The generic features of the dependence of the deflection angle on molecular weight, switching frequency, and electric field strength explain a number of experimental phenomena. DOI: 10.1103/PhysRevE.87.012723