Sub-100 nm Gold Nanoparticle Vesicles as a Drug Delivery Carrier enabling Rapid Drug Release upon Light Irradiation
Sub-100 nm Gold Nanoparticle Vesicles as a Drug Delivery Carrier enabling Rapid Drug Release upon Light Irradiation
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DOI：
10.1021/am400590m
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发表时间：
2013-05-08
影响因子：
9.5
通讯作者：
Ijiro, Kuniharu
中科院分区：
文献类型：
--
作者：
Niikura, Kenichi;Iyo, Naoki;Ijiro, Kuniharu
在此之前，我们报道了在四氢呋喃（THF）中自组装成直径小于100 nm的金纳米粒子囊泡（AuNVs）的半氟化配体包覆的金纳米粒子。(1)虽然这种尺寸对于体内使用是潜在有用的，但是AuNV的生物医学应用是有限的，因为囊泡结构在水中塌陷。在本文中，我们证明了金纳米粒子可以分散在水中，通过交联每个金纳米粒子与硫醇封端的PEG，使交联的囊泡可以作为药物递送载体，使光触发释放。若丹明染料或抗癌药物通过加热至62.5 ℃包封在交联囊泡内。在该温度下，纳米颗粒之间的间隙打开，如通过等离子体峰的蓝移和比在室温下观察到的更有效的包封所证实的。交联的AuNV在短期激光照射（5分钟，532 nm）后通过再次打开囊泡中每个纳米颗粒之间的纳米间隙释放包封的药物。相反，当将溶液加热至70 ℃时，包封染料的释放速度比激光照射引发的释放速度低得多（超过2小时），表明交联的AuNV对光具有高度响应性。与离散的金纳米颗粒相比，囊泡被有效地内化到细胞中，并在细胞中激光照射后释放抗癌药物。这些结果表明，尺寸小于100纳米的交联金纳米粒子可能是一种新型的光响应药物输送载体，适用于生物医学领域。
Previously, we reported gold nanoparticles coated with semi-fluorinated ligands self-assembled into gold nanoparticle vesicles (AuNVs) with a sub-100 nm diameter in tetrahydrofuran (THF).(1) Although this size is potentially useful for in vivo use, the biomedical applications of AuNVs were limited, as the vesicular structure collapsed in water. In this paper, we demonstrate that the AuNVs can be dispersed in water by cross-linking each gold nanoparticle with thiol-terminated PEG so that the cross-linked vesicles can work as a drug delivery carrier enabling light-triggered release. Rhodamine dyes or anticancer drugs were encapsulated within the cross-linked vesicles by heating to 62.5 degrees C. At this temperature, the gaps between nanoparticles open, as confirmed by a blue shift in the plasmon peak and the more efficient encapsulation than that observed at room temperature. The cross-linked AuNVs released encapsulated drugs upon short-term laser irradiation (5 min, 532 nm) by again opening the nanogaps between each nanoparticle in the vesicle. On the contrary, when heating the solution to 70 degrees C, the release speed of encapsulated dyes was much lower (more than 2 h) than that triggered by laser irradiation, indicating that cross-linked AuNVs are highly responsive to light. The vesicles were efficiently internalized into cells compared to discrete gold nanoparticles and released anticancer drugs upon laser irradiation in cells. These results indicate that cross-linked AuNVs, sub-100 nm in size, could be a new type of light-responsive drug delivery carrier applicable to the biomedical field.