IDENTIFICATION OF NEIGHBORING PROTEINS IN RIBOSOMES OF ESCHERICHIA-COLI - TOPOGRAPHICAL STUDY WITH CROSSLINKING REAGENT DIMETHYL SUBERIMIDATE
IDENTIFICATION OF NEIGHBORING PROTEINS IN RIBOSOMES OF ESCHERICHIA-COLI - TOPOGRAPHICAL STUDY WITH CROSSLINKING REAGENT DIMETHYL SUBERIMIDATE
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DOI：
10.1111/j.1432-1033.1974.tb03309.x
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发表时间：
1974-01-01
期刊：
影响因子：
--
通讯作者：
HAYES, D
中科院分区：
文献类型：
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作者：
CLEGG, C;HAYES, D
用二甲基亚胺酸二甲酯（一种针对蛋白质氨基的特异性交联剂）处理大肠杆菌的 30-S 和 50-S 核糖体亚基，导致形成新的蛋白质种类，可通过十二烷基硫酸钠中的丙烯酰胺凝胶电泳检测到。这些新物种被证明含有两个或多个连接在一起的核糖体蛋白，并且在用氨裂解交联后，通过二维电泳鉴定了这些成分。
Treatment of both the 30‐S and 50‐S ribosomal subunits ofEscherichia coliwith dimethyl suberimidate, a cross‐linking agent specific for protein amino groups, resulted in the formation of new protein species detectable by acrylamide gel electrophoresis in sodium dodecylsulfate. These new species were shown to contain two or more ribosomal proteins linked together and, after cleaving the cross‐links with ammonia, these constituents were identified by two‐dimensional electrophoresis.