Enzyme-mediated initiation of acrylamide polymerization:: reaction mechanism
Enzyme-mediated initiation of acrylamide polymerization:: reaction mechanism
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DOI：
10.1016/s0032-3861(00)00204-4
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发表时间：
2000-11-01
期刊：
影响因子：
4.6
通讯作者：
Maréchal, E
中科院分区：
文献类型：
--
作者：
Durand, A;Lalot, T;Maréchal, E
研究了辣根过氧化物酶催化氧化还原体系引发丙烯酰胺水溶液聚合。它结合过氧化氢（作为氧化剂）和2，4-戊二酮（作为还原剂）。通过聚合实验和UV光谱法证明了导致酶通过形成化合物III降解的几种副反应;它们可以通过调节反应物浓度来避免。2，4-戊二酮通过非酶途径的氧化也通过H-1 NMR检测到。其主要作用是降低烯醇形式的2，4-戊二酮的浓度，从而改变引发速率。根据这些信息，绘制了酶催化氧化还原体系中所涉及的反应的示意图，从而优化聚合条件。(C)2000爱思唯尔科技有限公司版权所有。
The polymerization of acrylamide in water initiated by a horseradish peroxidase-catalyzed redox system is studied. It combines hydrogen peroxide (as the oxidant) and 2,4-pentanedione (as the reductant). Several side reactions leading to the degradation of the enzyme through the formation of compound III are evidenced by polymerization experiments and UV spectroscopy; they can be avoided by adjusting the reactant concentrations. The oxidation of 2,4-pentanedione by a non-enzymic pathway is also detected by H-1 NMR. Its main effect is to reduce the concentration of the enol form of 2,4-pentanedione, and hence to modify the initiation rate. From this information, a schematic picture of the reactions involved in the enzyme-catalyzed redox system is drawn, so as to optimize the polymerization conditions. (C) 2000 Elsevier Science Ltd. All rights reserved.