Environmental temperature affects physiology and survival of nanosecond pulsed electric field-treated cells
Environmental temperature affects physiology and survival of nanosecond pulsed electric field-treated cells
复制标题
环境温度影响纳秒脉冲电场处理细胞的生理和存活
DOI：
10.1002/jcp.25984
复制
发表时间：
2018
影响因子：
5.6
通讯作者：
Wen Hao
中科院分区：
文献类型：
--
作者：
Yin Shengyong;Miao Xudong;Zhang Xueming;Chen Xinhua;Wen Hao
纳秒脉冲电场(NsPEF)是一种新型的非热肿瘤消融技术。然而，nsPEF在不同环境温度下如何影响细胞生理仍是个未知数。但这一问题与临床实践密切相关。这项工作旨在研究nsPEF处理的癌细胞对不同环境温度(0、4、25和37°C)的反应。检测细胞存活率、细胞凋亡率、线粒体膜电位和活性氧(ROS)。温度越低，凋亡率越高，线粒体膜电位越低，ROS水平越高。蔗糖和N-乙酰半胱氨酸(NAC)预先孵育可抑制ROS的生成并提高细胞存活率，从而保护nsPEF处理的细胞免受低温引起的细胞死亡。本工作为麻醉下行nsPEF消融术中的低温和输液提供了实验依据。
Nanosecond pulsed electric field (nsPEF) is a novel non‐thermal tumor ablation technique. However, how nsPEF affect cell physiology at different environmental temperature is still kept unknown. But this issue is of critical clinical practice relevance. This work aim to investigate how nsPEF treated cancer cells react to different environmental temperatures (0, 4, 25, and 37°C). Their cell viability, apoptosis, mitochondrial membrane potential, and reactive oxygen species (ROS) were examined. Lower temperature resulted in higher apoptosis rate, decreased mitochondria membrane potential, and increased ROS levels. Sucrose and N‐acetylcysteine (NAC) pre‐incubation inhibit ROS generation and increase cell survival, protecting nsPEF‐treated cells from low temperature‐caused cell death. This work provides an experimental basis for hypothermia and fluid transfusion during nsPEF ablation with anesthesia.