Production of human c-myc protein in insect cells infected with a baculovirus expression vector
Production of human c-myc protein in insect cells infected with a baculovirus expression vector
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在杆状病毒表达载体感染的昆虫细胞中产生人c-myc蛋白
DOI：
10.1128/mcb.5.10.2860-2865.1985
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发表时间：
1985
影响因子：
5.3
通讯作者：
G. Ju
中科院分区：
文献类型：
--
作者：
C. Miyamoto;G. Smith;J. Farrell;R. Chizzonite;M. Summers;G. Ju
将编码人c-myc的基因片段插入到杆状病毒加州链球线虫核型多角体病毒基因组的强多角体蛋白启动子附近。感染重组病毒的昆虫细胞产生大量的c-myc蛋白，这是这些细胞中的主要磷蛋白成分。通过免疫沉淀和免疫印迹分析，用c-myc特异性抗血清检测了61和两种蛋白。将昆虫来源的蛋白与在大肠杆菌和酿酒酵母细胞中合成的重组人c-myc编码蛋白进行了比较。C-myc基因产物主要是通过对受感染的昆虫细胞进行亚细胞分离而在细胞核中发现的。
A cDNA fragment coding for human c-myc was inserted into the genome of the baculovirus Autographa californica nuclear polyhedrosis virus adjacent to the strong polyhedrin promoter. Insect cells infected with the recombinant virus produced significant amounts of c-myc protein, which constituted the major phosphoprotein component in these cells. By immunoprecipitation and immunoblot analysis, two proteins of 61 and 64 kilodaltons were detected with c-myc-specific antisera. The insect-derived proteins were compared with recombinant human c-myc-encoded proteins synthesized in Escherichia coli and Saccharomyces cerevisiae cells. The c-myc gene product was found predominantly in the nucleus by subcellular fractionation of infected insect cells.
DOI：
10.1073/pnas.81.24.7742
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
RAMSAY, G;EVAN, GI;BISHOP, JM
通讯作者：
BISHOP, JM