Fetal brain subdivisions defined by R- and E-cadherin expressions: Evidence for the role of cadherin activity in region-specific, cell-cell adhesion
Fetal brain subdivisions defined by R- and E-cadherin expressions: Evidence for the role of cadherin activity in region-specific, cell-cell adhesion
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DOI：
10.1006/dbio.1995.8029
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发表时间：
1995-12-01
影响因子：
2.7
通讯作者：
Takeichi, M
中科院分区：
文献类型：
--
作者：
Matsunami, H;Takeichi, M
我们发现，R-钙粘蛋白，钙依赖性细胞间粘附分子，表达在小鼠胎脑的限制区域，如先前发现的E-钙粘蛋白。R-cadherin描绘了前脑神经节和某些未来的核团内的一个亚组，而E-cadherin则以另一种独特的模式表达。当细胞从胎儿脑的各个局部区域收集，解离，并在只有钙粘蛋白对细胞聚集有活性的条件下重新聚集时，R-钙粘蛋白阳性和阴性细胞彼此分离。对于E-钙粘蛋白获得了类似的结果。然而，这种分离的细胞被抑制时，钙粘蛋白失活的Ca 2+耗竭或阻断抗体。这些结果表明，钙粘蛋白赋予胎儿脑细胞的区域特异性染色，这一过程可能参与脑分割。(C)出版社：Academic Press
We found that R-cadherin, a Ca2+-dependent cell-cell adhesion molecule, is expressed in restricted regions of the mouse fetal brain, as was found for E-cadherin previously. R-cadherin delineated a subset of alar domains within forebrain neuromeres and certain future nuclei, while E-cadherin tvas expressed in another distinctive pattern. When cells were collected from various local regions of the fetal brain, dissociated, and reaggregated under the conditions in which only cadherins are active for cell aggregation, R-cadherin-positive and -negative cells segregated from one another. Similar results were obtained for E-cadherin. Such segregation of cells was, however, suppressed when the cadherins were inactivated either by Ca2+ depletion or with blocking antibodies. These results suggest that cadherins confer region-specific adhesiveness on fetal brain cells and that this process may take part in brain segmentation. (C) 1995 Academic Press, Inc.