Horseradish peroxidase: a valuable tool in biotechnology
Horseradish peroxidase: a valuable tool in biotechnology
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DOI：
10.1016/s1387-2656(03)09003-3
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发表时间：
2003-01-01
期刊：
影响因子：
--
通讯作者：
Fonseca, LP
中科院分区：
文献类型：
--
作者：
Azevedo, AM;Martins, VC;Fonseca, LP
过氧化物酶在生物技术及其相关研究领域(酶学、生物化学、医学、遗传学、生理学、组织学和细胞化学)中占有重要地位。它们是研究最广泛的酶类之一，文献中有丰富的研究论文，可以追溯到19世纪。尽管如此，过氧化物酶仍然被广泛研究，2002年已经发表了2000多篇文章(根据科学信息研究所的数据)。过氧化物酶在生物中的广泛分布和多种生理作用强调了过氧化物酶的重要性。根据它们的来源和作用方式，它们被分为三个超家族：植物过氧化物酶、动物过氧化物酶和过氧化氢酶。在所有的过氧化物酶中，辣根过氧化物酶(HRP)受到了特别的关注，并将成为本文的重点。本综述的第一部分包括对这三个超级家族的简要描述。第二部分对HRP的结构和催化作用的知识现状进行了全面的描述。第三节介绍了过氧化物酶在高等植物中的生理作用。最后，第四部分论述了过氧化物酶，特别是HRP在环境和卫生保健部门以及在制药、化学和生物技术行业中的应用。
Peroxidases have conquered a prominent position in biotechnology and associated research areas (enzymology, biochemistry, medicine, genetics, physiology, histo-and cytochem-istry). They are one of the most extensively studied groups of enzymes and the literature is rich in research papers dating back from the 19th century. Nevertheless, peroxidases continue to be widely studied, with more than 2000 articles already published in 2002 (according to the Institute for Scientific Information).The importance of peroxidases is emphasised by their wide distribution among living organisms and by their multiple physiological roles. They have been divided into three superfamilies according to their source and mode of action: plant peroxidases, animal peroxidases and catalases. Among all peroxidases, horseradish peroxidase (HRP) has received a special attention and will be the focus of this review. A brief description of the three super-families is included in the first section of this review. In the second section, a comprehensive description of the present state of knowledge of the structure and catalytic action of HRP is presented. The physiological role of peroxidases in higher plants is described in the third section. And finally, the fourth section addresses the applications of peroxidases, especially HRP, in the environmental and health care sectors, and in the pharmaceutical, chemical and biotechnological industries.