Attachment of the ubiquitin-related protein Urm1p to the antioxidant protein Ahp1p
Attachment of the ubiquitin-related protein Urm1p to the antioxidant protein Ahp1p
复制标题
DOI：
10.1128/ec.2.5.930-936.2003
复制
发表时间：
2003-10-01
期刊：
影响因子：
--
通讯作者：
Sprague, GF
中科院分区：
文献类型：
--
作者：
Goehring, AS;Rivers, DM;Sprague, GF
Urm 1 p是一种泛素相关蛋白，可作为其他蛋白的翻译后修饰。urm 1 p共轭已牵连在萌芽过程中，并在营养传感。在这里，我们已经确定了urmylation途径的第一个体内靶点为抗氧化蛋白Ahp 1 p。Urm 1 p与Ahp 1 p的连接需要用于urmylation途径的El，Uba 4p。urmylation途径的组成部分的损失的结果在巯基特异性氧化剂的敏感性，因为没有损失的Ahp 1 p，这意味着urmylation有一个氧化应激反应中的作用。此外，用硫醇特异性氧化剂处理细胞会影响Ahp 1 p-Urm 1 p缀合物的丰度。这些结果表明Urm 1 p与Ahp 1 p的结合可以调节Ahp 1 p在酿酒酵母抗氧化应激反应中的功能。
Urm1p is a ubiquitin-related protein that serves as a posttranslational modification of other proteins. Urm1p conjugation has been implicated in the budding process and in nutrient sensing. Here, we have identified the first in vivo target for the urmylation pathway as the antioxidant protein Ahp1p. The attachment of Urm1p to Ahp1p requires the El for the urmylation pathway, Uba4p. Loss of the urmylation pathway components results in sensitivity to a thiol-specific oxidant, as does loss of Ahp1p, implying that urmylation has a role in an oxidative-stress response. Moreover, treatment of cells with thiol-specific oxidants affects the abundance of Ahp1p-Urm1p conjugates. These results suggest that the conjugation of Urm1p to Ahp1p could regulate the function of Ahp1p in antioxidant stress response in Saccharomyces cerevisiae.