GENERATION OF A HUMAN X-DERIVED MINICHROMOSOME USING TELOMERE-ASSOCIATED CHROMOSOME FRAGMENTATION
GENERATION OF A HUMAN X-DERIVED MINICHROMOSOME USING TELOMERE-ASSOCIATED CHROMOSOME FRAGMENTATION
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DOI：
10.1002/j.1460-2075.1995.tb00228.x
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发表时间：
1995-11-01
期刊：
影响因子：
11.4
通讯作者：
COOKE, HJ
中科院分区：
文献类型：
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作者：
FARR, CJ;BAYNE, RAL;COOKE, HJ
线性哺乳动物人工染色体载体需要至少三个功能元件：一个着丝粒、两个端粒和复制起点，产生这样一个载体的一种方法是通过对现有染色体的碎裂。我们先前已经描述了使用克隆的端粒DNA来产生并稳定地在体细胞杂交中拯救人类X染色体的截断的衍生品。更多轮与端粒相关的染色体碎裂现已被用于设计一种人类X衍生的微型染色体。这个微小的染色体估计是
A linear mammalian artificial chromosome vector will require at least three functional elements: a centromere, two telomeres and replication origins, One route to generate such a vector is by the fragmentation of an existing chromosome, We have previously described the use of cloned telomeric DNA to generate and stably rescue truncated derivatives of a human X chromosome in a somatic cell hybrid. Further rounds of telomere-associated chromosome fragmentation have now been used to engineer a human X-derived minichromosome. This minichromosome is estimated to be