Electrodeposition polymers as immobilization matrices in amperometric biosensors: improved polymer synthesis and biosensor fabrication
Electrodeposition polymers as immobilization matrices in amperometric biosensors: improved polymer synthesis and biosensor fabrication
复制标题
电沉积聚合物作为电流生物传感器中的固定基质：改进的聚合物合成和生物传感器制造
DOI：
10.1007/s00216-009-3052-x
复制
发表时间：
2009
影响因子：
4.3
通讯作者：
W. Schuhmann
中科院分区：
文献类型：
--
作者：
D. Guschin;Halyna Shkil;W. Schuhmann
电沉积聚合物可通过电化学诱导调节电极前扩散区的pH值而沉积在电极表面上。所形成的聚合物膜可用作电流型生物传感器中的固定基质。为了合理地控制由此获得的生物传感器特性，开发用于电沉积聚合物的可再现合成的策略以及用于非手动和可再现的传感器制造的方法是必不可少的。基于仪器的发展，如一个专门设计的并行合成器，改进搅拌和温度控制，自动移液机器人的单体混合物的制备和控制去除阻聚剂，可重复的电沉积聚合物库的合成实现。此外，可以使用在线热电偶监测聚合过程，并且可以表明所选择的策略导致可再现的聚合反应。通过适应的电化学机器人系统集成的Au微量滴定板和自动电极清洁的抛光轮的装置，可再现的生物传感器制造使用葡萄糖氧化酶作为模型酶可以证明。这些结果为合理开发生物传感器和通过选择专门设计的电沉积聚合物来控制传感器性能开辟了途径。
图一个集成热电偶的并联合成器被设计用于控制同时8种电沉积聚合物的可重复合成。电化学机器人系统，然后用于自动制造的葡萄糖生物传感器和评价其性能。
AbstractElectrodeposition polymers can be precipitated on electrode surfaces upon electrochemical-induced modulations of the pH value in the diffusion zone in front of the electrode. The formed polymer films can be used as immobilization matrices in amperometric biosensors. In order to rationally control the thus obtained biosensor properties, it is indispensable to develop strategies for the reproducible synthesis of electrodeposition polymers as well as methods for the non-manual and reproducible sensor fabrication. Based on instrumental developments such as a specifically designed parallel synthesizer with improved stirring and temperature control, an automatic pipetting robot for the preparation of the monomer mixtures and controlled removal of polymerization inhibitors, the reproducible synthesis of libraries of electrodeposition polymers was achieved. Moreover, the polymerization process could be monitored using in-line thermocouples, and it could be shown that the chosen strategies led to reproducible polymerization reactions. By adaptation of an electrochemical robotic system integrating a Au microtiter plate and automatic electrode cleaning by means of a polishing wheel reproducible biosensor fabrication using glucose oxidase as a model enzyme could be demonstrated. These results open the route for the rational development of biosensors and control of the sensor properties by choosing specifically designed electrodeposition polymers.
FigureA parallel synthesizer with integrated thermoelements was designed for controlling the reproducible synthesis of simultaneously 8 electrodeposition polymers. An electrochemical robotic system was then employed for automatic fabrication of glucose biosensors and evaluation of their properties.