Polyelectrolyte complexes between (cross-linked) N-carboxyethylchitosan and (quaternized) poly[2-(dimethylamino)ethyl methacrylate]: preparation, characterization, and antibacterial properties.
Polyelectrolyte complexes between (cross-linked) N-carboxyethylchitosan and (quaternized) poly[2-(dimethylamino)ethyl methacrylate]: preparation, characterization, and antibacterial properties.
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DOI：
10.1021/bm061029j
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发表时间：
2007-02
影响因子：
6.2
通讯作者：
E. Yancheva;D. Paneva;V. Maximova;L. Mespouille;P. Dubois;N. Manolova;Iliya B Rashkov
中科院分区：
文献类型：
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作者：
E. Yancheva;D. Paneva;V. Maximova;L. Mespouille;P. Dubois;N. Manolova;Iliya B Rashkov
合成了N-羧乙基壳聚糖（CECh）与聚甲基丙烯酸二甲氨基乙酯（PDMAEMA）的新型季铵化复合物（佩奇）。将壳聚糖改性为CECh允许在壳聚糖不能形成复合物的pH范围内制备佩奇。CECh/PDMAEMA复合物在约7的窄pH范围内形成。PDMAEMA的叔氨基的季铵化使得能够在中性和碱性介质中与CECh形成络合物。交联的CECh也能够与（季铵化的）PDMAEMA形成复合物。评价了（交联）CECh、（季铵化）PDMAEMA及其复合物对大肠杆菌的抗菌活性。与（季铵化）PDMAEMA相反，（交联）CECh不显示抗菌活性。交联CECh和（季铵化）PDMAEMA之间的复合物形成导致后者在中性介质中固有的抗菌活性的损失。在酸性介质中，由于配合物的分解和（季铵化）PDMAEMA的释放，配合物表现出很强的抗菌活性。
Novel polyelectrolyte complexes (PECs) between N-carboxyethylchitosan (CECh) and well-defined (quaternized) poly[2-(dimethylamino)ethyl methacrylate] (PDMAEMA) have been obtained. The modification of chitosan into CECh allows the preparation of PECs in a pH range in which chitosan cannot form complexes. The CECh/PDMAEMA complex is formed in a narrow pH range around 7. The quaternization of the tertiary amino groups of PDMAEMA enables complex formation with CECh both in neutral and in alkaline medium. Cross-linked CECh is also capable of forming complexes with (quaternized) PDMAEMA. The antibacterial activity of (cross-linked) CECh, (quaternized) PDMAEMA, and their complexes against Escherichia coli has been evaluated. In contrast to (quaternized) PDMAEMA, (cross-linked) CECh exhibits no antibacterial activity. The complex formation between cross-linked CECh and (quaternized) PDMAEMA results in a loss of the inherent antibacterial activity of the latter in neutral medium. In acidic medium, the complexes exhibit strong antibacterial activity due to complex disintegration and release of (quaternized) PDMAEMA.