THE NUCLEAR MATRIX - A HEURISTIC MODEL FOR INVESTIGATING GENOMIC ORGANIZATION AND FUNCTION IN THE CELL-NUCLEUS
THE NUCLEAR MATRIX - A HEURISTIC MODEL FOR INVESTIGATING GENOMIC ORGANIZATION AND FUNCTION IN THE CELL-NUCLEUS
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DOI：
10.1002/jcb.240470204
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发表时间：
1991-10-01
影响因子：
4
通讯作者：
BEREZNEY, R
中科院分区：
文献类型：
--
作者：
BEREZNEY, R
尽管在破译基因组功能背后的分子事件方面取得了重大进展，但直到最近，我们对功能细胞核内这些整合过程的理解才取得了非常有限的成功。一个主要的难题是所有细胞结构和功能的“复杂层”特征。要了解细胞核的功能，我们还必须了解细胞核是如何组合在一起并作为一个整体发挥作用的。近年来在确定与核基质有关的各种核功能方面取得的巨大进展，证明了这种新整体方法的价值。在这篇文章中，我们总结了在原位核结构，孤立的核基质系统，核基质相关的功能，特别是DNA复制的基本特性。重点放在确定当前的问题和研究方向在这一领域，并说明这种全球性的方法基因组组织和功能的内在启发式价值。
Despite significant advances in deciphering the molecular events underlying genomic function, our understanding of these integrated processes inside the functioning cell nucleus has, until recently, met with only very limited success. A major conundrum has been the "layers of complexity" characteristic of all cell structure and function. To understand how the cell nucleus functions, we must also understand how the cell nucleus is put together and functions as a whole. The value of this neo-holistic approach is demonstrated by the enormous progress made in recent years in identifying a wide variety of nuclear functions associated with the nuclear matrix. In this article we summarize basic properties of in situ nuclear structure, isolated nuclear matrix systems, nuclear matrix-associated functions, and DNA replication in particular. Emphasis is placed on identifying current problems and directions of research in this field and illustrating the intrinsic heuristic value of this global approach to genomic organization and function.