Role of surface charge and wettability on early stage mineralization and bone cell-materials interactions of polarized hydroxyapatite
Role of surface charge and wettability on early stage mineralization and bone cell-materials interactions of polarized hydroxyapatite
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DOI：
10.1016/j.actbio.2009.02.023
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发表时间：
2009-07-01
影响因子：
9.7
通讯作者：
Bandyopadhyay, Amit
中科院分区：
文献类型：
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作者：
Bodhak, Subhadip;Bose, Susmita;Bandyopadhyay, Amit
我们的目的是确定表面电荷和润湿性在早期矿化以及骨细胞在极化的HAP表面上的黏附和增殖中的作用。为了评估表面润湿性，测量了在水、模拟体液(SBF)和Dulbecco的改进的Eagle‘s介质/营养混合物F-12火腿(DMEM)中的接触角。实验结果表明，HAP的表面润湿性和表面能可以通过诱导表面电荷来调节，而不会在材料中引入任何体积效应。增加表面电荷增加了HAP在所有被测介质中的润湿性和表面能。在400℃极化的正电荷HAP表面的最大表面能为49.47+/-3.76MJ/m(2)。极化的HAP样品通过浸泡在SBF和DMEM(细胞介质)中来评价其体外生物活性。通过与人胎儿成骨细胞(HFOB)的培养，研究细胞与材料的相互作用。体外实验结果表明，通过对HAP表面的润湿性和电荷极性的综合作用，可以使无机离子(如：Ca~(2+)、Cl~-、Na~+、HCO3-等)与具有不同表面性质的有机细胞黏附蛋白(如：纤维粘连蛋白、玻璃连结蛋白等)发生不同程度的结合，从而加速或减速矿化以及细胞在极化HAP表面的黏附和增殖。(C)2009年Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Our objective was to determine the role of surface charge and wettability on early stage mineralization as well as bone cell adhesion and proliferation on polarized HAp surface. To estimate the surface wettability, contact angles were measured in water, simulated body fluid (SBF) and Dulbecco's modified Eagle's medium/nutrient mixture F-12 Ham (DMEM). Experimental results show that HAp surface wettability and surface energy can be tailored by inducing surface charge without introducing any volumetric effects in the material. Increasing the surface charge increased the wettability and also the energy of HAp surfaces in all tested media. A maximum surface energy of 49.47 +/- 3.76 mJ/m(2) was estimated for positively charged HAp surfaces polarized at 400 degrees C. The in vitro bioactivity of polarized HAp samples was evaluated by soaking in SBF and DMEM (cell media). Cell-materials interaction was studied by culturing with human fetal osteoblast cells (hFOB). In vitro results show that tailoring the combined effect of wettability and charge polarity on the HAp surface enable differential binding of inorganic ions (e.g., Ca2+, Cl-, Na+, HCO3- etc) and organic cell adhesive proteins (e.g., fibronectin, vitronectin etc) with different surface properties, which results in accelerated or decelerated mineralization as well as cell adhesion and proliferation on polarized HAp surface. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.