CpLEPA is critical for chloroplast protein synthesis under suboptimal conditions in Arabidopsis thaliana.
CpLEPA is critical for chloroplast protein synthesis under suboptimal conditions in Arabidopsis thaliana.
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CpLEPA 对于拟南芥次优条件下的叶绿体蛋白质合成至关重要
DOI：
10.1371/journal.pone.0049746
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhang LX
中科院分区：
文献类型：
--
作者：
Ji DL;Lin H;Chi W;Zhang LX
LEPA是最保守的翻译因子之一，从细菌到高等植物都存在。然而，叶绿体LEPA同源物在高等植物中的生理功能仍然未知。在此，我们证明了cpLEPA在高等植物中实现高效光合作用的生理作用。cplepa-1突变体在正常生长条件下显示出稍高的叶绿素荧光和淡绿色表型。当在土壤上生长时，cplepa-1突变体的生长减少，并且在强光照射下观察到更大的减少。在cplepa-1突变体中，光合活性受损，这反映在叶绿体蛋白质的稳态水平降低。体内蛋白质标记实验解释了叶绿体蛋白质稳态水平的下降。叶绿体编码的mRNA与核糖体的异常关联表明，cplepa-1中的蛋白质合成缺陷是由于叶绿体中翻译起始的缺陷。cpLEPA蛋白似乎是一个重要的翻译因子，促进叶绿体蛋白合成的效率。
LEPA is one of the most conserved translation factors and is found from bacteria to higher plants. However, the physiological function of the chloroplast LEPA homolog in higher plants remains unknown. Herein, we demonstrate the physiological role of cpLEPA in enabling efficient photosynthesis in higher plants. The cplepa-1 mutant displays slightly high chlorophyll fluorescence and pale green phenotypes under normal growth conditions. The growth of the cplepa-1 mutant is reduced when grown on soil, and greater reduction is observed under intense light illumination. Photosynthetic activity is impaired in the cplepa-1 mutants, which is reflected in the decreased steady-state levels of chloroplast proteins. In vivo protein labeling experiments explained the decrease in the steady-state levels of chloroplast proteins. An abnormal association of the chloroplast-encoded mRNAs with ribosomes suggests that the protein synthesis deficiencies in cplepa-1 are due to defects in translation initiation in the chloroplasts. The cpLEPA protein appears to be an essential translation factor that promotes the efficiency of chloroplast protein synthesis.
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影响因子：
3.5
作者：
Aronsson, H;Jarvis, P
通讯作者：
Jarvis, P
影响因子：
4
作者：
BENDICH, AJ
通讯作者：
BENDICH, AJ
影响因子：
11.6
作者：
Meurer, J;Lezhneva, L;Oelmüller, R
通讯作者：
Oelmüller, R
DOI：
10.1073/pnas.0801308105
发表时间：
2008-03-25
影响因子：
11.1
作者：
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通讯作者：
Steitz, Thomas A.
影响因子：
7.4
作者：
Cai, Wenhe;Ji, Daili;Zhang, Lixin
通讯作者：
Zhang, Lixin