An Automated Assay to Measure Phagocytosis of Cryptococcus neoformans.
An Automated Assay to Measure Phagocytosis of Cryptococcus neoformans.
复制标题
测量新生隐球菌吞噬作用的自动测定。
DOI：
10.1002/cpmc.79
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发表时间：
2019
影响因子：
--
通讯作者：
Doering,TamaraL
中科院分区：
文献类型：
--
作者：
Chang,AndrewL;Hole,CamaronR;Doering,TamaraL
Cryptococcus neoformansis an opportunistic fungal pathogen that causes meningoencephalitis, which kills 200,000 individuals worldwide each year. It is ubiquitous in the environment and is first inhaled into the lungs of the host, where it is taken up by phagocytes. The interaction of these fungal cells with host phagocytes, therefore, is a critical step in the pathogenesis of this disease. One characteristic of this initial step in host–pathogen interactions is the avidity with which fungal cells are taken up by phagocytes, described by the phagocytic index. In this chapter, we detail a high‐throughput method of directly assessing the phagocytic index of fungal cells using an imaging‐based paradigm. By automating image collection and processing, this method permits rapid assessment of this critical host interaction. © 2019 by John Wiley & Sons, Inc.
