Histological brain alterations following prenatal methamphetamine exposure in rats
Histological brain alterations following prenatal methamphetamine exposure in rats
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DOI：
10.1111/j.1741-4520.2006.00126.x
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发表时间：
2006-12-01
影响因子：
1.3
通讯作者：
Fukui, Yoshihiro
中科院分区：
文献类型：
--
作者：
Cui, Chun;Sakata-Haga, Hiromi;Fukui, Yoshihiro
当孕妇滥用甲基苯丙胺时，最令人担忧的是这种物质对胎儿发育的潜在不利影响。对人类的临床研究发现，在大脑发育期间接触甲基苯丙胺会导致神经行为异常，如攻击性行为、学习问题和社交适应能力差。在本研究中，我们研究了胎儿期接触甲基苯丙胺对大鼠脑发育的影响。第一组孕鼠在孕期给予甲基苯丙胺5 mg/kg/d(GD10~GD20[MA])。第二组只注射生理盐水[SAL]。GD21天取胎，取胎脑进行观察。我们在MA胎脑中发现了各种类型的形态损害，包括小脑回、异位和出血。在某些病例中，除了这些组织学异常外，还观察到软脑膜的异常分布，如破裂或堆积。因此，我们检测了作为软脑膜重要组成部分的层粘连蛋白在胎儿大脑中的表达。免疫印迹分析显示，MA组和SAL组胎儿全脑层粘连蛋白的表达量无明显差异。我们得出的结论是，怀孕期间使用甲基苯丙胺会导致胎儿大脑的组织学变化。胎儿期接触甲基苯丙胺后脑组织的形态改变可能与宫内接触甲基苯丙胺的患者的神经行为异常有关。
When pregnant women abuse methamphetamine, the foremost concern is the potential adverse effect of this substance on fetal development. Clinical studies in humans have found that exposure to methamphetamine during brain development can cause neurobehavioral abnormalities, such as aggressive behavior, learning problems, and poor social adaptation. In the present study, we examined the effects of prenatal methamphetamine exposure on brain development in rats. The first group of pregnant rats was administered methamphetamine at a dose of 5 mg/kg/day during gestational day (GD 10 to GD 20 [MA]. The second group of pregnant rats was injected with saline vehicle only [SAL]. On GD 21 their fetuses were removed and fetal brains were observed. We found various types of morphological damage in MA fetal brains, including microgyria, ectopia, and hemorrhage. In some cases, abnormal distribution of the leptomeninx, such as breach or accumulation, was observed in addition to these histological abnormalities. Therefore, we examined the expression of laminin, which is an important component of the pia mater, in the fetal brains. However, Western blot analysis revealed that there was no difference in expression amount of laminin in whole fetal brain between the MA and SAL groups. We concluded that methamphetamine use during pregnancy can cause histological brain alterations in fetuses. Morphological alterations of brain seen in the present study and previous human studies following prenatal exposure to methamphetamine might be related to the neurobehavioral abnormalities seen in patients who had been exposed to methamphetamine in utero.