Comparative characterization of a wool-wax extract by two complementary chromatographic techniques.
Comparative characterization of a wool-wax extract by two complementary chromatographic techniques.
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通过两种互补色谱技术对羊毛蜡提取物进行比较表征。
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
J. Bayona
中科院分区：
文献类型：
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作者：
E. Jover;C. Domínguez;P. Erra;J. Bayona
一个详细的表征存在于羊毛蜡提取物，通过超临界流体萃取，提出。薄层色谱法耦合到火焰离子化检测器（TLC-FID）已被用作筛选技术，以确定复杂的混合物的不同化学类别。此外，为了确认通过TLC-FID获得的峰鉴别并检查其作为质量控制技术的适用性，通过重新分析通过TLC-FID和气相色谱法在电子碰撞和化学电离模式下与质谱联用获得的不同馏分，进行了制备型TLC分馏。通过比较研究，确定了薄层色谱-氢火焰离子化检测器鉴别方法。此外，所用不同技术的互补性证实了不同脂质类别的鉴定，即脂肪酸、醇、甾醇、甾烷二醇、甾烷酮、甾醇硫酸酯、羟基酸、烷二醇、脂肪族酯和甾醇酯、羟基甾醇酯、三酰甘油、二酯和II型和VI型神经酰胺。
A detailed characterization of lipids present in a wool-wax extract, obtained by supercritical fluid extraction, is presented. Thin-layer chromatography coupled to a flame ionization detector (TLC-FID) has been used as a screening technique to identify the different chemical classes of complex mixtures. Furthermore, to confirm peak identification obtained by TLC-FID and to check its suitability as a quality control technique, preparative TLC fractionation has been carried out by re-analyzing the different fractions obtained by TLC-FID and by gas chromatography coupled to mass spectrometry in the electron impact and in the chemical ionization modes. By means of this comparative study, the TLC-FID identification has been confirmed. Also, the complementarity of the different techniques used confirmed the identification of different lipid classes, namely fatty acids, alcohols, sterols, sterane diol, stanone, steryl sulphate, hydroxyacids, alkane diols, aliphatic and steryl esters, hydroxy steryl esters, triacylglycerol, diesters, and ceramides types II and VI.