Investigation of plant extracts in traditional medicine of the Brazilian Cerrado against protozoans and yeasts
Investigation of plant extracts in traditional medicine of the Brazilian Cerrado against protozoans and yeasts
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DOI：
10.1016/j.jep.2010.06.011
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发表时间：
2010-08-19
影响因子：
5.4
通讯作者：
Espindola, Laila Salmen
中科院分区：
文献类型：
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作者：
Albernaz, Lorena Carneiro;de Paula, Jose Elias;Espindola, Laila Salmen
研究目的：调查巴西Cerrado的传统医学中的217种植物提取物对原生动物和酵母菌的活性。材料和方法：用不同极性的溶剂浸渍的方法制备植物提取物。用氚标记次黄嘌呤掺入法测定抗氯喹恶性疟原虫FcB_1株的生长抑制作用。通过MTT比色测定来测量针对恰加斯利什曼原虫（Leishmania）和克氏锥虫的活性。采用CLSI方法进行抗真菌试验。结果：217种植物提取物对恶性疟原虫有较强的抗疟活性。评价了属于8种植物的11种活性提取物对L（L）chagasi、克氏锥虫、酵母和NIH-3 T3细胞的作用。在这些生物模型中发现的结果与这些植物的民族药理学数据一致。柿根的乙酸乙酯提取物对恶性疟原虫的IC 50值为1 μ g/mL。该浸提液对哺乳动物细胞无毒性，显著选择性指数（SI）为435.8。巴西红厚壳根木的二氯甲烷提取物对加特隐球菌LMGO 01具有活性，MIC为1.95 μ g/mL;白色念珠菌ATCC 10231和克鲁斯念珠菌LMGO 174均具有MIC为7.81 μ g/mL。相同的提取物对恶性疟原虫和恰加斯乳杆菌也具有活性，IC 50分别为6.7和27.6 μ g/mL。结果表明，盘龙参叶乙酸乙酯提取物对隐球菌LMGO 01的MIC为31.25 μ g/mL，对恶性疟原虫和克氏锥虫的IC 50分别为9.2 μ g/mL和56.3 μ g/mL。原生动物和人类致病酵母的活性提取物被认为有希望继续寻找鉴定和开发先导化合物。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Aim of the study: To investigate the activities of the 217 plant extracts in traditional medicine of the Brazilian Cerrado against protozoans and yeasts.Materials and methods: Plant extracts were prepared by the method of maceration using solvents of different polarities. The growth inhibition of chloroquine-resistant Plasmodium falciparum strain (FcB1) was determined by measuring the radioactivity of the tritiated hypoxanthine incorporated. Activity against Leishmania (Leishmania) chagasi and Trypanosoma cruzi was measured by the MTT colorimetric assay. The antifungal tests were carried out by using the CLSI method. The active extracts were tested also by cytotoxicity assay using NIH-3T3 cells of mammalian fibroblasts.Results: Two hundred and seventeen extracts of plants were tested against Plasmodium falciparum. The eleven active extracts, belonging to eight plant species were evaluated against L (L) chagasi, Trypanosoma cruzi, yeasts and in NIH-3T3 cells. The results found in these biological models are consistent with the ethnopharmacological data of these plants. The ethyl acetate extract of Diospyros hispida root showed IC50 values of 1 mu g/mL against Plasmodium falciparum. This extract demonstrated no toxicity against mammalian cells, resulting in a significant selectivity index (SI) of 435.8. The dichloromethane extract of Calophyllum brasiliense root wood was active against Cryptococcus gattii LMGO 01 with MIC of 1.95 mu g/mL; and Candida albicans ATCC 10231 and Candida krusei LMGO 174, both with MIC of 7.81 mu g/mL The same extract was also active against Plasmodium falciparum and L (L) chagasi with IC50 of 6.7 and 27.6 mu g/mL respectively. The ethyl acetate extract of Spiranthera odoratissima leaves was active against Cryptococcus gattii LMGO 01 with MIC of 31.25 mu g/mL, and against Plasmodium falciparum with IC50 of 9.2 mu g/mL and Trypanosoma cruzi with IC50 of 56.3 mu g/mLConclusion: The active extracts for protozoans and human pathogenic yeasts are considered promising to continue the search for the identification and development of leading compounds. (C) 2010 Elsevier Ireland Ltd. All rights reserved.