Methods in cell separations
Methods in cell separations
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DOI：
10.1007/10_2007_069
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Mattiasson, Bo
中科院分区：
文献类型：
--
作者：
Dainiak, Maria B.;Kumar, Ashok;Mattiasson, Bo
细胞生物学和生物医学领域的研究依赖于将细胞群破碎并将稀有细胞类型分离至高纯度的技术。简要概述了目前用于细胞分离的方法和市售产品。细胞分级的大小和密度和高度选择性的亲和为基础的技术，如亲和层析，荧光激活细胞分选（FACS）和磁性细胞分选的吞吐量，产量和纯度方面进行了讨论。
Research in the field of cell biology and biomedicine relies on technologies that fractionate cell populations and isolate rare cell types to high purity. A brief overview of methods and commercially available products currently used in cell separations is presented. Cell fractionation by size and density and highly selective affinity-based technologies such as affinity chromatography, fluorescence-activated cell sorting (FACS) and magnetic cell sorting are discussed in terms of throughput, yield, and purity.