Transcriptional regulation of a brown adipocyte-specific gene, UCP1, by KLF11 and KLF15
Transcriptional regulation of a brown adipocyte-specific gene, UCP1, by KLF11 and KLF15
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DOI：
10.1016/j.bbrc.2010.08.039
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发表时间：
2010-09-10
影响因子：
3.1
通讯作者：
Huh, Nam-ho
中科院分区：
文献类型：
--
作者：
Yamamoto, Ken-ichi;Sakaguchi, Masakiyo;Huh, Nam-ho
一些生长因子和转录因子在棕色脂肪细胞分化和产热基因表达调控中发挥重要作用，尤其是UCP1的表达。在本研究中，我们重点研究了在棕色脂肪组织中高表达的KLF11和KLF15。我们的数据表明，KLF11和KLF15分别通过GC-box和gt -box直接与UCP1启动子相互作用。棕色脂肪细胞分化过程中，在间充质干细胞系muBM3.1中共转染KLF11和KLF15可提高UCP1的表达水平。在muBM3.1棕色脂肪细胞分化过程中，KLF11对UCP1的表达至关重要，而不是KLF15。(C) 2010爱思唯尔公司版权所有。
Several growth factors and transcription factors have been reported to play important roles in brown adipocyte differentiation and modulation of thermogenic gene expression, especially the expression of UCP1. In this study, we focused on KLF11 and KLF15, which were expressed highly in brown adipose tissue. Our data demonstrated that KLF11 and KLF15 interacted directly with the UCP1 promoter using GC-box and GT-boxes, respectively. Co-transfection of KLF11 and KLF15 in the mesenchymal stem cell line muBM3.1 during brown adipocyte differentiation enhanced the expression level of UCP1. KLF11, but not KLF15, was essential for UCP1 expression during brown adipocyte differentiation of muBM3.1. (C) 2010 Elsevier Inc. All rights reserved.