Elevation of CRABP-I in the cerebrospinal fluid of patients with moyamoya disease
Elevation of CRABP-I in the cerebrospinal fluid of patients with moyamoya disease
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DOI：
10.1161/01.str.0000100159.43123.d7
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发表时间：
2003-12-01
期刊：
影响因子：
8.3
通讯作者：
Wang, KC
中科院分区：
文献类型：
--
作者：
Kim, SK;Yoo, JI;Wang, KC
背景和目的--烟雾病(MMD)的病因尚不清楚。方法：对20例经血管造影证实的MMD患者(男4例，女16例；年龄3~13岁，平均7.5岁)和4例脑瘫患者(男2例，女2例；年龄5~10岁，平均7.3岁)的脑脊液进行研究。脑脊液蛋白采用双向聚丙烯酰胺凝胶电泳法分析，蛋白质鉴定采用基质辅助激光解吸/电离飞行时间质谱仪。Western blotting证实MMD患者脑脊液中存在特异性蛋白。结果：我们鉴定出1个多肽斑点(M-r大小为13~15 kDa，等电点为5~5.5)，在MMD患者的脑脊液标本中有差异表达(平均光密度为0.36+/-0.24；范围为0.05~0.92)；对照组的脑脊液标本中有差异表达(平均为0.03+/-0.04；范围为0~0.08；P=0.002)。Western blotting证实17例MMD患儿脑脊液中存在CRABP-I的高表达。本研究提示脑脊液中CRABP-I的升高可能是MMD发病机制之一。
Background and Purpose - The etiology of moyamoya disease ( MMD) remains obscure. This study was undertaken to identify specific proteins associated with the pathogenesis of MMD.Methods - We studied cerebrospinal fluid ( CSF) from 20 patients with angiographically confirmed MMD ( 4 boys and 16 girls; age range, 3 to 13 years; mean, 7.5 years) and 4 control patients with cerebral palsy who underwent selective dorsal rhizotomy ( 2 boys and 2 girls; age range, 5 to 10 years; mean, 7.3 years). CSF proteins were analyzed by 2- dimensional polyacrylamide gel electrophoresis, and protein identification was performed by matrix- assisted laser desorption/ ionization time- of- flight mass spectrometry. The presence of specific CSF protein in patients with MMD was confirmed by Western blotting. In addition, cerebral CSF was also tested in 7 patients who had other brain diseases but no MMD ( 2 boys and 5 girls; age range, 1 to 12 years; mean, 6.9 years).Results - We identified 1 polypeptide spot ( M-r of 13 to 15 kDa and isoelectric point of 5 to 5.5) that was differentially expressed in the CSF samples of MMD patients ( mean optical density intensity, 0.36 +/- 0.24; range, 0.05 to 0.92) and control spinal CSF samples ( mean, 0.03 +/- 0.04; range, 0 to 0.08; P = 0.002). This polypeptide was identified as cellular retinoic acid - binding protein ( CRABP)- I. High levels of expression of CRABP- I in the CSF from 17 MMD children were confirmed by Western blotting.Conclusions - The analysis of the CSF of MMD patients reveals high CRABP- I expression. The present study suggests that the elevation of CRABP- I in CSF may be a candidate for pathogenesis of MMD.