Effect of poly(ethylene glycol) molecular weight on tensile and swelling properties of oligo(poly(ethylene glycol) fumarate) hydrogels for cartilage tissue engineering
Effect of poly(ethylene glycol) molecular weight on tensile and swelling properties of oligo(poly(ethylene glycol) fumarate) hydrogels for cartilage tissue engineering
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DOI：
10.1002/jbm.1259
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发表时间：
2002-03-05
期刊：
影响因子：
--
通讯作者：
Mikos, AG
中科院分区：
文献类型：
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作者：
Temenoff, JS;Athanasiou, KA;Mikos, AG
本研究旨在确定聚乙二醇（PEG）分子量的变化对由我们实验室最近开发的新型聚合物聚（聚（乙二醇）富马酸酯）（OPF）制成的水凝胶的膨胀和机械性能的影响。考察了初始PEG分子量为860、3900和9300的OPF水凝胶的性能。PEG 3900配方的拉伸模量为23.1 +/- 12.4 kPa，断裂伸长率为53.2 +/- 13.7%；PEG 9300配方具有相似的拉伸性能（模量：16.5 +/-4.6 kPa，伸长率：76.0 +/- 26.4%）。然而，与其他配方相比，PEG 860凝胶具有更高的模量（89.5 +/- 50.7 kPa）和更小的断裂伸长率（30.1 +/-6.4%）。此外，每个配方之间的肿胀百分比有显着差异。计算了每种OPF配方的交联分子量（M-c）和网目尺寸。PEG 9300的M-c浓度从2010 +/- 116 g/mol增加到6250 +/- 280 g/mol。网格尺寸计算显示了类似的趋势（PEG 860的76 +/-2埃到PEG 9300的160 +/-6埃）。还发现，如果在第一层水凝胶完全交联之前加入第二层水凝胶，这些水凝胶可以层压。这些层压凝胶的力学测试表明，界面区域的存在并没有显着改变其拉伸性能。这些结果表明，通过改变合成中所使用的PEG的分子量，可以改变OPF基水凝胶的材料性能，并且可以制备多层OPF水凝胶结构，每层具有不同的力学性能。(C) 2001约翰威利父子公司[J] .中国生物医学工程学报，2009,31(4):559 - 567。
This study was designed to determine the effect of changes in poly(ethylene glycol) (PEG) molecular weight on swelling and mechanical properties of hydrogels made from a novel polymer, oligo(poly(ethylene glycol) fumarate) (OPF), recently developed in our laboratory. Properties of hydrogels made from OPF with initial PEG molecular weights of 860, 3900, and 9300 were examined. The PEG 3900 formulation had a tensile modulus of 23.1 +/- 12.4 kPa and percent elongation at fracture of 53.2 +/- 13.7%; the PEG 9300 formulation had similar tensile properties (modulus: 16.5 +/-4.6 kPa, elongation: 76.0 +/- 26.4%). However, the PEG 860 gels had a significantly higher modulus (89.5 +/- 50.7 kPa) and a significantly smaller percent elongation at fracture (30.1 +/-6.4%), when compared with other formulations. Additionally, there were significant differences in percent swelling between each of the formulations. Molecular weight between crosslinks (M-c) and mesh size were calculated for each OPF formulation. M-c increased from 2010 +/- 116 g/mol with PEG 860 to 6250 +/- 280 g/mol with PEG 9300. Mesh size calculations showed a similar trend (76 +/-2 Angstrom for PEG 860 to 160 +/-6 Angstrom for PEG 9300). It was also found that these hydrogels could be laminated if a second layer was added before the first had completely crosslinked. Mechanical testing of these laminated gels revealed that the presence of an interfacial area did not significantly alter their tensile properties. These results suggest that the material properties of OPF-based hydrogels can be altered by changing the molecular weight of PEG used in synthesis and that multilayered OPF hydrogel constructs can be produced, with each layer having distinct mechanical properties. (C) 2001 John Wiley & Sons, Inc. J Biomed Mater Res 59: 429-437, 2002.