Enzyme-containing silica inverse opals prepared by using water-soluble colloidal crystal templates: Characterization and application
Enzyme-containing silica inverse opals prepared by using water-soluble colloidal crystal templates: Characterization and application
复制标题
使用水溶性胶体晶体模板制备含酶二氧化硅反蛋白石：表征与应用
DOI：
10.1016/j.bej.2016.04.007
复制
发表时间：
2016-08-15
影响因子：
3.9
通讯作者：
Gao, Jing
中科院分区：
文献类型：
--
作者：
Jiang, Yanjun;Zheng, Pengju;Gao, Jing
以水溶性聚丙烯酰胺(PAM)胶体晶体为模板，首次制备了含酶二氧化硅反蛋白石。首次制备了含葡萄糖氧化酶的SiO_2反蛋白石(GOD@SiO_3)，并用扫描电子显微镜、电子显微镜、激光共聚焦显微镜和氮气吸附-脱附等手段对其进行了表征。与自由的上帝相比，上帝@SIO对极端的pH、热和化学变性剂更稳定。GOD@SIOS可用于快速、灵敏的葡萄糖目视检测。为了验证该方法适用于各种酶，还制备了含有有机磷水解酶(OPH)的二氧化硅反蛋白石，并成功地将其应用于有机磷化合物的视觉和光谱检测。这些含有酶的二氧化硅反蛋白石为原位固定化生物分子提供了一种新的方法。(C)2016年，由爱思唯尔出版。
Enzyme-containing silica inverse opals are prepared for the first time by using water-soluble polyacrylamide (PAM) colloidal crystals as templates. Glucose oxidase-containing silica inverse opals (GOD@SIOs) are firstly fabricated and characterized by SEM, TEM, CLSM, and N-2 adsorption-desorption. Compared with free GOD, the GOD@SIOs are more stable against extreme pH, heat, and chemical denaturants. GOD@SIOs can be used in fast and sensitive visual detection of glucose. In order to verify that this method is suitable for various enzymes, organophosphorus hydrolase (OPH)-containing silica inverse opals are also prepared and successfully applied as sensors for visual and spectrometric detection of organophosphorus based compounds. These enzyme-containing silica inverse opals offer a novel approach to in situ immobilize biomolecules. (c) 2016 Published by Elsevier B.V.