Varying allergen composition and content affects the in vivo allergenic activity of commercial Dermatophagoides pteronyssinus extracts.
Varying allergen composition and content affects the in vivo allergenic activity of commercial Dermatophagoides pteronyssinus extracts.
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DOI：
10.1159/000337654
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发表时间：
2012
影响因子：
2.8
通讯作者：
Vrtala S
中科院分区：
文献类型：
--
作者：
Casset A;Mari A;Purohit A;Resch Y;Weghofer M;Ferrara R;Thomas WR;Alessandri C;Chen KW;de Blay F;Valenta R;Vrtala S
屋尘螨（HDM）过敏的诊断和免疫治疗仍然基于天然过敏原提取物。本研究的目的是分析来自不同制造商的市售屋尘螨提取物的过敏原组成和含量，以及变化是否会影响其过敏原活性。对几种D.使用屋尘螨变应原（Der p 1、2、5、7、10和21）通过免疫印迹分析来自10个不同制造商的提取物。夹心ELISA用于定量提取物中的Der p 1和Der p 2。螨过敏患者（n = 45）用浸提液进行皮肤试验，并通过斑点印迹法检测免疫球蛋白E（IgE）对10种螨过敏原（Der p 1、2、4、5、7、8、10、14、20和21）的反应性。在所有提取物中仅检测到Der p 1和Der p 2，但其浓度和比例显示出高度变异性（Der p 1：6.0-40.8 μg ml−1; Der p 2：1.7-45.0 μg ml−1）。在8种研究的浸提液中未检测到4种过敏原中的至少1种（即Der p 5、7、10和21）。螨过敏受试者对单个螨过敏原表现出不同的IgE反应性特征，提取物在皮肤点刺试验和假阴性结果中表现出不同的过敏活性。市售D.屋盖蕨提取物缺乏重要的过敏原，在过敏原组成和含量方面显示出很大的可变性，并且一些在某些患者中给出假阴性诊断测试结果。
Diagnosis and immunotherapy of house-dust mite (HDM) allergy is still based on natural allergen extracts. The aim of this study was to analyze commercially available Dermatophagoides pteronyssinus extracts from different manufacturers regarding allergen composition and content and whether variations may affect their allergenic activity. Antibodies specific for several D. pteronyssinus allergens (Der p 1, 2, 5, 7, 10 and 21) were used to analyze extracts from 10 different manufacturers by immunoblotting. Sandwich ELISAs were used to quantify Der p 1 and Der p 2 in the extracts. Mite-allergic patients (n = 45) were skin-tested with the extracts and tested for immunoglobulin E (IgE) reactivity to a panel of 10 mite allergens (Der p 1, 2, 4, 5, 7, 8, 10, 14, 20 and 21) by dot blot. Only Der p 1 and Der p 2 were detected in all extracts but their concentrations and ratios showed high variability (Der p 1: 6.0–40.8 μg ml−1; Der p 2: 1.7–45.0 μg ml−1). At least 1 out of 4 allergens (i.e. Der p 5, 7, 10 and 21) was not detected in 8 of the studied extracts. Mite-allergic subjects showed different IgE reactivity profiles to the individual mite allergens, the extracts showed different allergenic activity in skin-prick tests and false-negative results. Commercially available D. pteronyssinus extracts lack important allergens, show great variability regarding allergen composition and content and some gave false-negative diagnostic test results in certain patients.