Simultaneous Measurement of Ionic Current and Fluorescence from Single Protein Pores
Simultaneous Measurement of Ionic Current and Fluorescence from Single Protein Pores
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DOI：
10.1021/ja808128s
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发表时间：
2009-02-11
影响因子：
15
通讯作者：
Wallace, Mark I.
中科院分区：
文献类型：
--
作者：
Heron, Andrew J.;Thompson, James R.;Wallace, Mark I.
同时监测膜通道和膜孔的离子电流和荧光的能力有可能将这种蛋白质的结构变化与功能联系起来。我们提出了一种利用油包水滴Mayers同时测量膜蛋白的单通道电流和荧光的新方法。我们展示了在多个葡萄球菌α-溶血素小孔中同时检测环糊精随机封闭的荧光和电学检测。来自个体的组合荧光信号。孔洞显示出与总电流记录相同的阻塞事件序列，表明来自每个孔洞的两个信号是相关的。
The ability to simultaneously monitor both the ionic current and fluorescence from membrane channels and pores has the potential to link structural changes with function in such proteins. We present a new method for simultaneously measuring single-channel electrical currents and fluorescence from membrane proteins by using water-in-oil droplet Mayers. We demonstrate the simultaneous fluorescence and electrical detection of stochastic blocking by cyclodextrin in multiple staphylococcal alpha-hemolysin pores. The combined fluorescence signal from individual. pores exhibits the same sequence of blocking events as the total current recording, showing that the two signals from each pore are correlated.