Semi-micro ion chromatography of iodide in seawater
Semi-micro ion chromatography of iodide in seawater
复制标题
海水中碘化物的半微量离子色谱法
DOI：
10.1016/s0021-9673(98)00910-8
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发表时间：
1999
影响因子：
4.1
通讯作者：
Kazuaki Ito
中科院分区：
文献类型：
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作者：
Kazuaki Ito
采用柱上分析物聚焦技术和柱上基质消除技术对海水中痕量碘离子进行了半微离子色谱分析。采用半微柱（35×1 mm I.D.）填充高阴离子交换容量的苯乙烯-二乙烯基苯共聚物，流动相为0.03 M NaClO4+0.5 M NaCl+ 5mm磷酸钠缓冲液，pH为6.0。海水中的碘离子通过静电和疏水相互作用有效地富集在色谱柱上，洗脱时无峰展宽。流动相中的ClO4 - (NaClO4)对碘离子和Cl - (NaCl)的洗脱均有较好的效果，既能提高碘离子（I -）的疏水性，又能去除主要阴离子的干扰。海水中过量的主要阴离子不影响在UV - 226 nm下碘化物的检测。连续注射5和1 μg/l I−（2 ml 35‰人工海水）的相对标准偏差分别为1.5和5.8% （n=5）。使用半微柱的校准曲线斜率（按峰面积计算）比使用相同树脂（150×4.6 mm id）的传统柱高~ 2.8倍。该方法对35‰人工海水2 ml的检出限为0.2 μg/l I−，适用于海水样品。
Large sample volume injections including both on-column analyte focusing and on-column matrix elimination techniques were examined for semi-micro ion chromatography of trace iodide (I−) in seawater. A semi-microcolumn (35×1 mm I.D.) packed with styrene–divinylbenzene copolymer with high anion-exchange capacity and a mobile phase of 0.03 M NaClO4+0.5 M NaCl+5 mM sodium phosphate buffer, pH 6.0, was used. Iodide in seawater was effectively concentrated on the column by both electrostatic and hydrophobic interactions and was eluted without peak broadening. ClO4−(NaClO4) in the mobile phase was effective for the elution of iodide and Cl−(NaCl) for both the concentration of iodide (I−) with hydrophobicity and the removal of interference by the major anions. An excess of major anions in seawater did not disturb the detection of iodide at UV 226 nm. The relative standard deviations for successive injections of 5 and 1 μg/l I−(2 ml of 35‰ artificial seawater) were 1.5 and 5.8% (n=5, each), respectively. The slope of calibration curve (by peak area) using the semi-microcolumn was ∼2.8-times higher than that for a conventional column with the same resin (150×4.6 mm I.D.) The present method had a detection limit of 0.2 μg/l I−for 2 ml of 35‰ artificial seawater and was successfully applied to seawater samples.