Investigation of the anti-cataractogenic mechanisms of curcumin through in vivo and in vitro studies
Investigation of the anti-cataractogenic mechanisms of curcumin through in vivo and in vitro studies
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DOI：
10.1186/s12886-018-0711-8
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发表时间：
2018-02-17
影响因子：
2
通讯作者：
Wang, Meng
中科院分区：
文献类型：
--
作者：
Cao, Jing;Wang, Tao;Wang, Meng
背景：白内障是世界范围内老年人致盲的主要原因，尤其是在发展中国家。迫切需要研究确定可以预防或延缓白内障形成的策略。本研究旨在探讨姜黄素在体内和体外的细胞保护作用机制。方法：雄性Wistar大鼠随机分为3组：对照组、模型组和姜黄素组。用RT-PCR和ELISA法检测热休克蛋白70(HSP70)的表达水平、8-羟基-2-脱氧鸟苷(8-OHdG)、过氧化氢酶(CAT)、丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活性。体外实验采用CCK-8比色法和流式细胞仪检测细胞存活率、细胞凋亡率和细胞周期，RT-PCR检测caspase3、Bcl2相关X(Bax)、B细胞淋巴瘤2(Bcl2)、环氧合酶(COX 2)、c Met和Slug的mRNA表达水平。姜黄素组HSP70表达水平及8-OHdG、MDA活性低于模型组，CAT、SOD、GSH-Px活性显著高于模型组(P<0.05)。在体外实验中，姜黄素组与模型组相比，细胞存活率显著升高，细胞凋亡率显著降低。相应地，姜黄素组caspase-3、Bax和COX-2的mRNA表达低于模型组(P<；0.05)。结论：姜黄素可能通过减少细胞内活性氧的产生，保护细胞免受氧化损伤，从而减轻亚硒酸钠诱导的白内障。
Background: Cataract is the leading cause of blindness in elderly people worldwide, especially in developing countries. Studies to identify strategies that can prevent or retard cataract formation are urgently required. This study aimed to investigate the potential mechanism of the cytoprotective effects of curcumin in in vivo and in vitro experiments.Methods: Male Wistar rats were randomly divided into three groups: the control group, the model group (administered 20 mu mol/kg sodium selenite), and the curcumin group (pretreated with 75 mg/kg body weight curcumin 24 h prior to the administration of sodium selenite). The expression levels of heat shock protein 70 (HSP70), the activities of 8-hydroxy-2-deoxyguanosine (8-OHdG), catalase (CAT), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) were assessed by using RT-PCR assay and ELISA. In addition, the cell viability, cell apoptosis, and cell cycle were assessed using a CCK-8 assay and flow cytometry in in vitro studies, followed by RT-PCR analysis to identify the mRNA expression levels of caspase 3, Bcl-2 associated X (Bax), B-cell lymphoma 2 (Bcl-2), cyclooxygenase (Cox-2), c-met, and Slug.Results: Cataract was successfully established in rats of the model group and the curcumin group through intraperitoneal injection of sodium selenite. The expression levels of HSP70 and the activities of 8-OHdG and MDA in the curcumin group were decreased compared with those in the model group, whereas the activities of CAT, SOD, and GSH-Px were significantly higher than those in the model group (P < 0.05). In the in vitro studies, the cell viability and cell apoptosis significantly increased and decreased, respectively, in the curcumin group compared with the model group. Correspondingly, the mRNA expression of caspase-3, Bax, and Cox-2 was lower in the curcumin group than in the model group (P < 0.05).Conclusions: This study suggested that curcumin attenuated selenite-induced cataract through the reduction of the intracellular production of reactive oxygen species and the protection of cells from oxidative damage.