Polarization-sensitive optical coherence tomography for renal tumor detection in ex vivo human kidneys
Polarization-sensitive optical coherence tomography for renal tumor detection in ex vivo human kidneys
复制标题
用于离体人肾肾肿瘤检测的偏振敏感光学相干断层扫描
DOI：
10.1016/j.optlaseng.2023.107900
复制
发表时间：
2024
影响因子：
4.6
通讯作者：
Tang, Qinggong
中科院分区：
文献类型：
--
作者：
Yan, Feng;Wang, Chen;Yan, Yuyang;Zhang, Qinghao;Yu, Zhongxin;Patel, Sanjay G.;Fung, Kar-Ming;Tang, Qinggong
肾癌是一种高死亡率的恶性肿瘤，早期诊断困难，治疗时易发生转移。手术切除肿瘤是治疗肾癌最有效的方法。然而，在手术切除期间，精确评估肿瘤边缘是一个挑战。本研究的目的是展示一种光学成像工具，可以精确区分肾脏肿瘤边界，并从正常组织中识别肿瘤区域，以帮助外科医生在手术过程中准确切除肾脏肿瘤。使用偏振敏感光学相干断层扫描（PS-OCT）对来自6个人肾脏的30个样本进行成像。获得肾脏样品的横截面、表面和空间信息用于微环境重建。利用偏振参数（相位延迟、光轴方向和偏振均匀度（DOPU））和斯托克斯参数（Q、U和V）进行多参数分析。为了验证PS-OCT的检测准确性，利用H&E组织学染色和切片系数来量化PS-OCT在识别肿瘤边界和区域方面的性能。在本研究中，PS-OCT成像清晰地识别了肿瘤边界，优于传统的基于强度的OCT。以H&E组织学染色为金标准，PS-OCT基于偏振和Stokes参数精确地识别了不同位置和不同深度的肿瘤区域和组织分布。与传统的衰减系数量化方法相比，由于双折射效应，PS-OCT显示了正常组织和癌组织之间组织特征的增强对比。我们的研究结果表明，PS-OCT有望为肾脏肿瘤的手术切除提供成像指导，并有可能用于临床上的其他人类肾脏手术，如肾活检。
Kidney cancer is a kind of high mortality cancer because of the difficulty in early diagnosis and the high metastatic dissemination in treatments. The surgical resection of tumors is the most effective treatment for renal cancer patients. However, precise assessment of tumor margins is a challenge during surgical resection. The objective of this study is to demonstrate an optical imaging tool in precisely distinguishing kidney tumor borders and identifying tumor zones from normal tissues to assist surgeons in accurately resecting tumors from kidneys during the surgery. 30 samples from six human kidneys were imaged using polarization-sensitive optical coherence tomography (PS-OCT). Cross-sectional,enface, and spatial information of kidney samples were obtained for microenvironment reconstruction. Polarization parameters (phase retardation, optic axis direction, and degree of polarization uniformity (DOPU) and Stokes parameters (Q, U, and V) were utilized for multi-parameter analysis. To verify the detection accuracy of PS-OCT, H&E histology staining and dice-coefficient were utilized to quantify the performance of PS-OCT in identifying tumor borders and regions. In this study, tumor borders were clearly identified by PS-OCT imaging, which outperformed the conventional intensity-based OCT. With H&E histological staining as golden standard, PS-OCT precisely identified the tumor regions and tissue distributions at different locations and different depths based on polarization and Stokes parameters. Compared to the traditional attenuation coefficient quantification method, PS-OCT demonstrated enhanced contrast of tissue characteristics between normal and cancerous tissues due to the birefringence effects. Our results demonstrated that PS-OCT was promising to provide imaging guidance for the surgical resection of kidney tumors and had the potential to be used for other human kidney surgeries in clinics such as renal biopsy.