Metformin derived carbon dots: Highly biocompatible fluorescent nanomaterials as mitochondrial targeting and blood-brain barrier penetrating biomarkers
Metformin derived carbon dots: Highly biocompatible fluorescent nanomaterials as mitochondrial targeting and blood-brain barrier penetrating biomarkers
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DOI：
10.1016/j.jcis.2021.02.058
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发表时间：
2021-03-12
影响因子：
9.9
通讯作者：
Leblanc, Roger M.
中科院分区：
文献类型：
--
作者：
Cilingir, Emel Kirbas;Seven, Elif S.;Leblanc, Roger M.
碳点(Cd)自2004年被发现以来，因其具有低毒、良好的生物相容性、高的光致发光性和良好的水分散性等独特的性质而受到人们的广泛关注。本研究采用微波辅助方法合成了二甲双胍衍生碳点(Met-CDs)。利用紫外可见光谱(UV-Vis)、光致发光光谱(PL)、傅立叶变换红外光谱(FTIR)、X射线光电子能谱(XPS)、原子力显微镜(AFM)和透射电子显微镜(TEM)对MET-CDs进行了表征。细胞毒性研究结果表明，Met-CDs对非肿瘤细胞和肿瘤细胞都具有低毒和良好的生物相容性，是研究活细胞生物成像的优秀候选材料。此外，生物成像研究表明，Met-CDs可以穿透细胞膜，分散在包括细胞核和线粒体在内的整个细胞结构中。更具体地说，Met-CDs往往在孵育1小时后开始选择性地定位于癌细胞的线粒体内，而不是非肿瘤细胞的线粒体内。最后，斑马鱼的一项研究证实，Met-CDs可以在不需要任何其他配体的情况下穿过血脑屏障(BBB)。综上所述，本研究介绍了Met-CDs的合成，它具有线粒体和细胞核定位以及血脑屏障穿透的能力。(C)2021 Elsevier Inc.版权所有。碳点(CDs)自2004年发现以来，由于其独特的性质，如低毒、良好的生物相容性、高光致发光(PL)和良好的水分散性，一直受到广泛的研究。本研究采用微波辅助方法合成了二甲双胍衍生碳点(Met-CDs)。利用紫外可见光谱(UV-Vis)、光致发光光谱(PL)、傅立叶变换红外光谱(FTIR)、X射线光电子能谱(XPS)、原子力显微镜(AFM)和透射电子显微镜(TEM)对MET-CDs进行了表征。细胞毒性研究结果表明，Met-CDs对非肿瘤细胞和肿瘤细胞都具有低毒和良好的生物相容性，是研究活细胞生物成像的优秀候选材料。此外，生物成像研究表明，Met-CDs可以穿透细胞膜，分散在包括细胞核和线粒体在内的整个细胞结构中。更具体地说，Met-CDs往往在孵育1小时后开始选择性地定位于癌细胞的线粒体内，而不是非肿瘤细胞的线粒体内。最后，斑马鱼的一项研究证实，Met-CDs可以在不需要任何其他配体的情况下穿过血脑屏障(BBB)。综上所述，本研究合成了具有线粒体和细胞核定位以及血脑屏障穿透能力的Met-CDs。
Carbon dots (CDs) have been intensively studied since their discovery in 2004 because of their unique properties such as low toxicity, excellent biocompatibility, high photoluminescence (PL) and good water dispersibility. In this study metformin derived carbon dots (Met-CDs) were synthesized using a microwave assisted method. Met-CDs were meticulously characterized using ultra-violet spectroscopy (UV-vis), photoluminescence (PL), Fourier Transform Infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), atomic force (AFM) and transmission electron (TEM) microscopies. According to results of cytotoxicity studies, Met-CDs possess low-toxicity and excellent biocompatibility towards both non-tumor and tumor cell lines indicating that Met-CDs are outstanding candidates for living cell bioimaging studies. Furthermore, bioimaging studies have displayed that Met-CDs can penetrate the cell membrane and disperse throughout the cell structure including the nucleus and mitochondria. More specifically, Met-CDs tend to start localizing selectively inside the mitochondria of cancer cells, but not of non-tumor cells after 1 h of incubation. Finally, a zebrafish study confirmed that Met-CDs cross the blood-brain barrier (BBB) without the need of any other ligands. In summary, this study presents synthesis of Met-CDs which feature abilities such as mitochondrial and nucleus localizations along with BBB penetration.(c) 2021 Elsevier Inc. All rights reserved.Carbon dots (CDs) have been intensively studied since their discovery in 2004 because of their unique properties such as low toxicity, excellent biocompatibility, high photoluminescence (PL) and good water dispersibility. In this study metformin derived carbon dots (Met-CDs) were synthesized using a microwave assisted method. Met-CDs were meticulously characterized using ultra-violet spectroscopy (UV?vis), photoluminescence (PL), Fourier Transform Infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), atomic force (AFM) and transmission electron (TEM) microscopies. According to results of cytotoxicity studies, Met-CDs possess low-toxicity and excellent biocompatibility towards both non-tumor and tumor cell lines indicating that Met-CDs are outstanding candidates for living cell bioimaging studies. Furthermore, bioimaging studies have displayed that Met-CDs can penetrate the cell membrane and disperse throughout the cell structure including the nucleus and mitochondria. More specifically, Met-CDs tend to start localizing selectively inside the mitochondria of cancer cells, but not of non-tumor cells after 1 h of incubation. Finally, a zebrafish study confirmed that Met-CDs cross the blood?brain barrier (BBB) without the need of any other ligands. In summary, this study presents synthesis of Met-CDs which feature abilities such as mitochondrial and nucleus localizations along with BBB penetration.