A PSC101-DERIVED PLASMID WHICH SHOWS NO SEQUENCE HOMOLOGY TO OTHER COMMONLY USED CLONING VECTORS
A PSC101-DERIVED PLASMID WHICH SHOWS NO SEQUENCE HOMOLOGY TO OTHER COMMONLY USED CLONING VECTORS
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DOI：
10.1016/0378-1119(84)90207-5
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发表时间：
1984-01-01
期刊：
影响因子：
3.5
通讯作者：
NAGAMINE, Y
中科院分区：
文献类型：
--
作者：
CHURCHWARD, G;BELIN, D;NAGAMINE, Y
构建了源自大肠杆菌质粒pSC101的质粒克隆载体pGB2。该质粒对壮观霉素和链霉素具有抗性，包含 HindIII、PstI、SalI、BamHI、SmaI 和 EcoRI 酶的独特限制位点。通过DNA-DNA杂交检测，pGB2与几种广泛使用的载体如pBR322、pUC8和噬菌体λL47.1没有序列同源性。在其他应用中，DNA片段可以被克隆到质粒中，然后放射性质粒DNA可以用作探针来筛选重组DNA文库。
A plasmid cloning vector, pGB2, which is derived from the Escherichia coli plasmid pSC101 was constructed. The plasmid, which specifies resistance to spectinomycin and streptomycin, contains unique restriction sites for the enzymes HindIII, PstI, SalI, BamHI, SmaI, and EcoRI. pGB2 shows no sequence homology, as detected by DNA-DNA hybridization, to several widely used vectors such as pBR322, pUC8 and phage .lambda.L47.1. Among other applications, DNA fragments can be cloned into the plasmid and then radioactive plasmid DNA can be used as a probe to screen recombinant DNA libraries.