Isolation of hepatocyte stimulating factor from human monocytes.
Isolation of hepatocyte stimulating factor from human monocytes.
复制标题
从人单核细胞中分离肝细胞刺激因子。
DOI：
10.1016/s0006-291x(87)80063-3
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发表时间：
1987
影响因子：
3.1
通讯作者：
Nham,SU
中科院分区：
文献类型：
--
作者：
Fuller,GM;Bunzel,RJ;Woloski,BM;Nham,SU
肝细胞刺激因子是一种单核细胞衍生的蛋白质，调节肝血浆蛋白质的合成。采用离子交换、凝胶渗透和反相层析等方法从粘附的人外周血单核细胞中纯化人肝细胞刺激因子。电泳分析表明其Mr为28，000道尔顿，等电点为5.4。对肝细胞刺激因子和白细胞介素-1的特异性生物学测定表明，它们是不同的，具有独立的生物学效应。
Hepatocyte stimulating factor is a monocyte derived protein which regulates hepatic plasma protein synthesis. Human hepatocyte stimulating factor was purified to apparent homogeneity from adherent peripheral blood monocytes by using ion excahange, gel permeationa and reversed phase chromatography. Electrophoretic analysis showed that it has a Mrof 28,000 daltons and an isoelectric point of 5.4. Biological assays specific for heptocyte stimulating factor and interleukin-1 showed that they are distinct and have independent biological effects.